Description

Chloramphenicol Acetyltransferase (CAT) is a key bacterial enzyme that catalyzes the acetylation of chloramphenicol, rendering the antibiotic inactive. This product is essential for molecular biology research, serving as a critical reporter gene for studying gene expression and promoter activity in various cellular systems. It is primarily utilized by biotechnology firms, academic research institutions, and pharmaceutical R&D departments involved in genetic engineering, assay development, and antibiotic resistance studies.

Application

• Reporter Gene Assays: Widely used as a standard reporter enzyme in transient and stable transfection experiments to measure promoter strength and transcriptional regulation.
• Molecular Biology Research: A fundamental tool for studying gene expression mechanisms, signal transduction pathways, and genetic regulatory elements.
• Antibiotic Resistance Studies: Used to investigate and model bacterial resistance mechanisms against chloramphenicol and related antibiotics.
• Biotechnology & Pharmaceutical Development: Employed in high-throughput screening (HTS) platforms and the development of novel gene therapy vectors and expression systems.
• Diagnostic Kit Manufacturing: Serves as a component in enzymatic assay kits for research and diagnostic purposes.
• Educational & Training Purposes: Used in advanced biochemistry and molecular biology laboratory courses to demonstrate enzyme kinetics and gene expression analysis.

Basic Information

Product Name	Chloramphenicol Acetyltransferase
CAS No.	9040-07-7
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	CAT; Chloramphenicol Acetyl Transferase; Chloramphenicol O-Acetyltransferase; Acetyltransferase, Chloramphenicol; CAT Enzyme; 3.1.1.29; Chloramphenicol 3-O-Acetyltransferase
EINECS	Contact for details

Quality Control

Our Chloramphenicol Acetyltransferase is produced and tested under stringent quality management protocols to ensure high purity, specific activity, and batch-to-batch consistency suitable for sensitive research applications. Each lot is characterized for enzymatic activity and purity profile. A comprehensive Certificate of Analysis (COA) detailing assay results, purity, and stability data is provided with every shipment and is available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below for long-term stability. This material is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature before opening to minimize moisture absorption. For prolonged storage, consider under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder or solution
Identification (Activity)	Conforms
Specific Activity	≥ [Value] units/mg protein
Purity (SDS-PAGE)	≥ 95%
Protein Concentration	As per COA
Endotoxin Level	< 1.0 EU/μg (for cell culture grade)
Solubility	Completely soluble in appropriate assay buffer
Storage Buffer	Specified on COA

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.