Description

Phenylethanolamine n-Methyltransferase, Bovine, Adrenal is a purified enzyme preparation derived from bovine adrenal glands, specifically catalyzing the N-methylation of phenylethanolamine. This enzyme is a critical research tool for studying the biosynthesis of catecholamines and related neurotransmitters. It is essential for biochemical research, pharmaceutical development, and diagnostic applications where the investigation of adrenergic pathways is required.

Application

• Biochemical Research: Used as a key reagent in enzymatic assays to study the kinetics and mechanisms of phenylethanolamine N-methyltransferase (PNMT) activity.
• Neurotransmitter Synthesis Studies: Essential for in vitro studies investigating the final step in the biosynthesis of epinephrine (adrenaline) from norepinephrine.
• Pharmaceutical Screening: Employed in high-throughput screening platforms to identify and characterize potential inhibitors or modulators of PNMT for drug discovery.
• Diagnostic Development: Serves as a component in the development of enzymatic diagnostic kits for research into adrenal function and catecholamine-related disorders.
• Enzyme Characterization: Used for the purification, characterization, and structural analysis of PNMT to understand its role in physiological and pathological states.
• Educational Purposes: A standard material for advanced biochemistry and molecular biology laboratory courses focusing on enzymology and metabolic pathways.

Basic Information

Product Name	Phenylethanolamine n-Methyltransferase, Bovine, Adrenal
CAS No.	9037-68-7
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	PNMT; Phenylethanolamine N-Methyltransferase; Adrenal Phenylethanolamine N-Methyltransferase; Noradrenaline N-Methyltransferase; Epinephrine Synthetase; E.C. 2.1.1.28; S-Adenosyl-L-methionine:phenylethanolamine N-methyltransferase
EINECS	Contact for details

Quality Control

Our Phenylethanolamine n-Methyltransferase, Bovine, Adrenal is produced and tested under stringent quality protocols to ensure high purity, specific activity, and batch-to-batch consistency suitable for research applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing parameters such as protein concentration, specific activity, and purity assessed by SDS-PAGE. We adhere to rigorous in-house standards to guarantee product integrity and performance reliability.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below for long-term stability. This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to minimize exposure to atmospheric moisture. Avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	Lyophilized powder or solution
Identification (Activity)	Functional activity confirmed
Protein Content (Biuret)	≥ 90% of stated value
Specific Activity	Contact for details
Purity (SDS-PAGE)	≥ 90%
Solubility	Clear solution in specified buffer
pH (in solution)	7.0 - 8.0
Endotoxin (LAL)	< 1.0 EU/µg protein

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.