Description

δ-Aminolevulinate Dehydratase (ALAD) is a crucial zinc-binding metalloenzyme that catalyzes the second step in the heme biosynthesis pathway. Its reliable activity and purity are essential for consistent results in biochemical research, diagnostic assay development, and enzymatic studies. This product is primarily utilized by researchers and manufacturers in the life sciences, pharmaceutical, and in-vitro diagnostic (IVD) industries.

Application

• Biochemical Research: Used as a key reagent in studies of porphyrin and heme biosynthesis, enzyme kinetics, and metabolic pathway analysis.
• Diagnostic Assay Development: Serves as a critical component in the development of enzymatic assays for the detection of lead poisoning, as ALAD activity is a sensitive biomarker for lead exposure.
• Enzyme Activity Studies: Employed to investigate enzyme inhibition, metal cofactor requirements, and the biochemical basis of porphyrias and other related disorders.
• Pharmaceutical Screening: Used in high-throughput screening platforms to identify potential modulators or inhibitors of the heme biosynthesis pathway for therapeutic development.
• Reference Standard: Acts as a purified standard for quality control in the production of diagnostic kits and for calibrating laboratory equipment.

Basic Information

Product Name	δ-Aminolevulinate Dehydratase
CAS No.	9036-37-7
Molecular Formula	N/A (Protein/Enzyme)
Molecular Weight	Approx. 280-300 kDa (homooctamer) - Contact for details on specific isoform.
Synonyms	ALAD; Porphobilinogen Synthase; PBG Synthase; 5-Aminolevulinate Dehydratase; Aminolevulinic Acid Dehydratase; δ-Aminolevulinic Acid Dehydratase; ALADH; HemB; EC 4.2.1.24
EINECS	Contact for details

Quality Control

Our δ-Aminolevulinate Dehydratase is produced and tested under stringent conditions to ensure high enzymatic activity, purity, and batch-to-batch consistency. Each lot is characterized for specific activity, protein content, and absence of significant contaminating proteases. A comprehensive Certificate of Analysis (COA) detailing purity (by SDS-PAGE), specific activity (μmol PBG/hr/mg protein), pH optimum, and stability data is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store lyophilized powder at -20°C. Reconstituted enzyme solutions should be aliquoted and stored at -70°C or below to prevent loss of activity. The product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use and allowed to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	Lyophilized powder
Specific Activity	≥ 30 units/mg protein
Protein Content (Biuret)	≥ 90%
Purity (SDS-PAGE)	≥ 95%
pH Optimum	6.3 - 6.8
Stabilizers	May contain buffer salts (e.g., phosphate)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.