Description

Ec 4.2.1.2 CAS NO 9032-88-6 is a high-purity enzyme preparation, specifically a fumarate hydratase (fumarase) that catalyzes the reversible hydration of fumarate to L-malate. This biocatalyst is essential for critical biochemical synthesis and analytical processes, offering high specificity and activity. It is primarily utilized by manufacturers and research institutions in the pharmaceutical, biotechnology, and diagnostic reagent sectors for enzymatic synthesis, metabolic pathway studies, and biosensor development.

Application

• Pharmaceutical Intermediate Synthesis: Key catalyst in the enzymatic production of L-malic acid and related chiral intermediates.
• Biotechnological Production: Used in bioreactors and fermentation processes for the biosynthesis of organic acids.
• Diagnostic Reagent Formulation: A critical component in clinical diagnostic kits for the determination of fumarate or in coupled enzyme assays.
• Biochemical Research: Essential for in vitro metabolic studies, enzyme kinetics research, and teaching laboratory experiments.
• Food Ingredient Processing: Potential application in the production of food-grade acidulants.
• Biosensor Development: Immobilized enzyme for creating sensitive and specific biosensors in analytical chemistry.

Basic Information

Product Name	Ec 4.2.1.2 CAS NO 9032-88-6
CAS No.	9032-88-6
Molecular Formula	N/A (Enzyme)
Molecular Weight	~200,000 Da (Subunit composition dependent)
Synonyms	Fumarase; Fumarate Hydratase; L-Malate Hydro-Lyase; (S)-Malate Hydro-Lyase; EC 4.2.1.2; Fumarate Hydratase (Class I); Fumarase C; FumC; Fumarase A; FumA
EINECS	Contact for details

Quality Control

Our enzyme preparations are manufactured under strict quality management systems. Each batch of Fumarase (EC 4.2.1.2) is subjected to rigorous testing for specific activity, purity (via SDS-PAGE), and absence of contaminating protease or nuclease activities. Certificates of Analysis (COA) detailing activity units/mg protein, pH optimum, temperature stability, and other relevant parameters are provided with every shipment to ensure compliance with your research or production specifications.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below. This product is hygroscopic (moisture-sensitive). Allow the vial to reach room temperature before opening to minimize moisture condensation. For short-term use, store at 2-8°C. Avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder or solution
Identification (Activity)	Catalyzes the hydration of fumarate to L-malate
Specific Activity	≥ 300 units/mg protein (Contact for specific grade)
Protein Content (Biuret)	≥ 90% of total content
Purity (SDS-PAGE)	Single major band
pH Optimum	7.0 - 8.5
Temperature Optimum	37°C
Stabilizers	May contain glycerol or buffer salts

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.