Description

Cytochrome B(5) Reductase CAS NO 9032-25-1 is a critical flavoprotein enzyme that catalyzes the transfer of electrons from NADH to cytochrome b5, playing an essential role in cellular redox metabolism. This enzyme is vital for maintaining metabolic homeostasis, including the desaturation and elongation of fatty acids and the metabolism of drugs and xenobiotics. It is a key reagent for research institutions and pharmaceutical companies engaged in biochemical studies, metabolic pathway analysis, and the development of therapeutic agents targeting oxidative stress and metabolic disorders.

Application

• Biochemical Research: Fundamental studies on electron transport chains, redox biology, and enzyme kinetics.
• Pharmaceutical Development: Used in drug metabolism studies (Phase I reactions) and as a target for compounds addressing metabolic diseases.
• Diagnostic Reagent Manufacturing: A component in enzymatic assay kits for clinical diagnostics and research use.
• Metabolomics & Systems Biology: Investigation of lipid metabolism, including fatty acid desaturation pathways.
• Enzyme Engineering: Serves as a model protein for studies in protein structure, function, and stability.
• Academic & Industrial R&D: Essential for laboratories focusing on biochemistry, molecular biology, and cellular physiology.

Basic Information

Product Name	Cytochrome B(5) Reductase
CAS No.	9032-25-1
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	NADH-Cytochrome b5 Reductase; Cytochrome b5 Reductase; DIA1; b5R; Methemoglobin Reductase; NADH-Ferricytochrome b5 Oxidoreductase; EC 1.6.2.2
EINECS	Contact for details

Quality Control

Our Cytochrome B(5) Reductase is produced and tested under stringent quality management systems. Each batch undergoes rigorous analytical testing to ensure high purity, specific activity, and functional integrity. Certificates of Analysis (COA) detailing purity (by SDS-PAGE/HPLC), specific activity, and other critical parameters are available upon request to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below. This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent moisture absorption. Avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	Lyophilized powder or solution
Identification (Activity)	Conforms
Purity (SDS-PAGE)	≥ 90%
Specific Activity	≥ 50 units/mg protein
Protein Content (Bradford)	As per COA
Solubility	Clear in appropriate buffer

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.