Description

Diisopropylfluoro-Phosphatase, Lyo is a lyophilized (freeze-dried) enzyme preparation, specifically a phosphatase enzyme stabilized with diisopropyl fluorophosphate (DFP). This product matters for researchers and industrial biochemists requiring a precisely inhibited enzyme for control experiments, mechanistic studies, or the stabilization of enzyme preparations. It is needed in pharmaceutical research, diagnostic kit manufacturing, and academic biochemistry laboratories where the study of phosphatase activity and its specific inhibition is critical.

Application

• Biochemical Research: Used as a negative control in enzymatic assays to study the specific activity of serine phosphatases by comparison with DFP-inhibited samples.
• Diagnostic Reagents: Incorporated into control calibrators and assay kits to ensure specificity and accuracy in clinical diagnostic tests for phosphatase-related biomarkers.
• Enzyme Stabilization: Acts as a stabilizing agent in master mixes and long-term enzyme storage formulations by irreversibly inhibiting protease contamination.
• Pharmaceutical Development: Employed in drug discovery and development pipelines to investigate enzyme inhibition mechanisms and screen for potential therapeutic compounds.
• Cell Signaling Studies: Utilized in molecular biology to dissect phosphorylation/dephosphorylation pathways by selectively blocking specific phosphatase activities.
• Food & Beverage Analysis: Applied in analytical methods for the detection of phosphatase activity as an indicator of pasteurization efficacy or product quality.

Basic Information

Product Name	Diisopropylfluoro-Phosphatase, Lyo
CAS No.	9032-18-2
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	DFP-Phosphatase, Lyo; Diisopropyl Phosphorofluoridate-Inactivated Phosphatase; Phosphatase, DFP-treated; Serine Phosphatase Inhibitor (DFP); Alkaline Phosphatase, DFP-Inhibited; Acid Phosphatase, DFP-Inhibited; Lyophilized Fluorophosphatase; E.C. 3.1.3.x (DFP-Inhibited)
EINECS	Contact for details

Quality Control

Our Diisopropylfluoro-Phosphatase, Lyo is produced under a quality management system. Each batch undergoes rigorous analytical testing to ensure consistent activity profile, purity, and DFP inhibition efficacy. Certificates of Analysis (COA) detailing specific parameters such as residual activity, protein content, and lyophilization quality are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C. The product is hygroscopic (moisture-sensitive). For long-term stability, store desiccated at -20°C. Allow the vial to equilibrate to room temperature before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (IR)	Conforms to reference standard
Residual Phosphatase Activity	< 5% of uninhibited control
Protein Content (Biuret)	Reported on COA
Moisture Content (Karl Fischer)	< 5.0%
Solubility	Clear, slightly opalescent solution in specified buffer
Biological Activity (Post-Reconstitution)	Stable for specified period at 2-8°C

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.