Description

Isoleucine-Trna Ligase CAS NO 9030-96-0 is a critical aminoacyl-tRNA synthetase enzyme that catalyzes the esterification of isoleucine to its cognate tRNA, a fundamental step in protein biosynthesis. This enzyme is essential for ensuring translational fidelity and maintaining cellular homeostasis. It is primarily utilized in biochemical research, molecular biology studies, and pharmaceutical development, particularly in investigations related to protein synthesis, antibiotic mechanism of action, and enzyme-targeted drug discovery.

Application

• Biochemical Research & Enzyme Kinetics: Used as a model enzyme to study aminoacyl-tRNA synthetase specificity, catalytic mechanisms, and proofreading functions.
• Antibiotic Development & Screening: Serves as a target for screening and developing novel antimicrobial agents that inhibit bacterial protein synthesis.
• Molecular Biology Tool: Employed in in vitro translation systems and for the preparation of aminoacylated tRNAs for specialized research applications.
• Structural Biology Studies: Provides material for X-ray crystallography and cryo-EM to elucidate enzyme structure-function relationships.
• Diagnostic Reagent Component: May be incorporated into specialized diagnostic kits for research into genetic disorders linked to translational errors.
• Pharmaceutical R&D: Used in target validation and high-throughput screening campaigns within drug discovery pipelines.

Basic Information

Product Name	Isoleucine-Trna Ligase
CAS No.	9030-96-0
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Isoleucyl-tRNA synthetase; IleRS; Isoleucine--tRNA ligase; EC 6.1.1.5; L-Isoleucine:tRNA(Ile) ligase (AMP-forming); IARS; Isoleucinyl-tRNA Synthetase
EINECS	Contact for details

Quality Control

Our Isoleucine-Trna Ligase is produced and tested under stringent conditions to ensure high activity, purity, and batch-to-batch consistency suitable for research applications. Quality is verified through a panel of analytical methods including activity assays, SDS-PAGE, and HPLC analysis. A comprehensive Certificate of Analysis (COA) detailing specific activity, purity level, and buffer composition is provided with each shipment.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store frozen at -20°C or below. Avoid repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent moisture absorption and activity loss.

Specification

Item	Specification
Appearance	Clear to slightly opalescent solution
Identification (Activity)	Conforms
Specific Activity	≥ [Value] units/mg protein
Purity (SDS-PAGE)	≥ 90%
Protein Concentration	Provided on COA
Buffer Composition	Tris-HCl, Glycerol, DTT (details on COA)
Endotoxin Level	< 1.0 EU/μg (if applicable)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.