Description

4-Hydroxy-2-Oxoglutarate Aldolase, ~0.1 U/Mg* is a highly specific enzyme critical for catalyzing the aldol cleavage of 4-hydroxy-2-oxoglutarate to glyoxylate and pyruvate. This enzymatic activity is fundamental for research and development in metabolic pathway analysis and enzymology studies. It is an essential reagent for biochemical research laboratories, pharmaceutical R&D, and diagnostic kit manufacturers focusing on metabolic disorders and enzyme function assays.

Application

• Biochemical Research: A key tool for studying the glyoxylate and dicarboxylate metabolism pathways, enzyme kinetics, and reaction mechanisms.
• Pharmaceutical Development: Used in target validation and screening assays for drugs intended to modulate related metabolic processes.
• Diagnostic Reagent Manufacturing: Incorporated into enzymatic assay kits for the quantitative determination of specific metabolites in clinical and research samples.
• Metabolomics Studies: Employed to elucidate and quantify metabolic fluxes in cellular systems, tissues, or biofluids.
• Enzyme Engineering: Serves as a model enzyme for protein engineering studies aimed at improving stability, activity, or specificity.
• Academic & Industrial R&D: Essential for fundamental and applied research in biochemistry, molecular biology, and biotechnology.

Basic Information

Product Name	4-Hydroxy-2-Oxoglutarate Aldolase, ~0.1 U/Mg*
CAS No.	9030-81-3
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	4-Hydroxy-2-oxoglutarate aldolase; 2-Oxo-4-hydroxyglutarate aldolase; KHG aldolase; 2-Keto-4-hydroxyglutarate aldolase; 4-Hydroxy-2-ketoglutarate aldolase; Hydroxypyruvate aldolase; EC 4.1.3.16; 4-Hydroxy-2-oxoglutarate glyoxylate-lyase
EINECS	Contact for details

Quality Control

Our 4-Hydroxy-2-Oxoglutarate Aldolase is produced and tested under stringent quality management protocols to ensure batch-to-batch consistency and reliable enzymatic activity. Each lot is characterized for specific activity, purity, and stability. A comprehensive Certificate of Analysis (COA) detailing assay results, storage conditions, and stability data is provided with every shipment to support your quality assurance and regulatory needs.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C. This material is hygroscopic (moisture-sensitive) and should be handled under controlled humidity conditions to maintain stability and activity. For long-term storage, consider storing at -20°C in aliquots to avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	Lyophilized powder or solution
Specific Activity	~0.1 U/mg protein (or as per COA)
Protein Content	Determined by Biuret or Bradford assay
Purity (SDS-PAGE)	≥ 90%
Identification (Activity)	Catalyzes the cleavage of 4-hydroxy-2-oxoglutarate
pH Optimum	~7.0 - 8.0 (Phosphate buffer)
Stabilizers/Additives	May contain buffer salts (e.g., phosphate, Tris)
Solubility	Soluble in aqueous buffers

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.