Description

β-1,4-Galactosyl Transferase From Bovine Milk is a highly purified enzyme that catalyzes the transfer of galactose from UDP-galactose to acceptor molecules with a β-1,4 linkage. This specificity makes it an indispensable tool for the controlled synthesis and modification of oligosaccharides, glycoproteins, and glycolipids. It is essential for research and development in biochemistry, glycobiology, and the production of specialized bioactive compounds for the pharmaceutical and diagnostic industries.

Application

• Glycobiology Research: A critical reagent for studying glycosylation pathways, enzyme mechanisms, and carbohydrate-protein interactions.
• Oligosaccharide Synthesis: Used in the enzymatic synthesis of complex, structurally defined oligosaccharides and glycoconjugates for research and therapeutic applications.
• Glycoprotein Engineering: Enables the site-specific modification of proteins to alter their pharmacokinetic properties, stability, or biological activity.
• Diagnostic Reagent Production: Employed in the manufacturing of enzymatic assay kits and components for clinical diagnostics targeting carbohydrate-related biomarkers.
• Biocatalysis: Utilized in green chemistry approaches for the stereoselective synthesis of chiral intermediates and fine chemicals.
• Nutritional Science Studies: Applied in research to understand the biosynthesis of human milk oligosaccharides (HMOs) and develop novel nutritional ingredients.

Basic Information

Item	Details
Product Name	β-1,4-Galactosyl Transferase From Bovine Milk
CAS No.	9030-11-9
Molecular Formula	Protein
Molecular Weight	~50-55 kDa (monomer)
Synonyms	β-1,4-Galactosyltransferase; β4Gal-T1; UDP-Galactose:N-Acetylglucosamine β-1,4-Galactosyltransferase; Lactose Synthase (A protein); N-Acetyllactosamine Synthase; B4GALT1; EC 2.4.1.38; Bovine Milk Galactosyltransferase
EINECS	Contact for details

Quality Control

Our β-1,4-Galactosyl Transferase is produced and tested under stringent quality management systems. Each lot undergoes comprehensive analysis to ensure high specific activity, purity, and functional consistency. A detailed Certificate of Analysis (COA) is provided, documenting key parameters such as activity, protein content, and absence of contaminating enzymatic activities, supporting research reproducibility and compliance.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below. The product is hygroscopic (moisture-sensitive); allow the vial to equilibrate to room temperature before opening to prevent condensation. Aliquotting is recommended to avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	Lyophilized powder or solution
Specific Activity	> 10 units/mg protein
Protein Content (Biuret)	> 90%
Purity (SDS-PAGE)	> 95%
Identification (Activity)	Conforms
pH (in solution)	6.5 - 7.5
Endotoxin	< 1.0 EU/μg

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.