Description

Sarcosine Oxidase CAS NO 9029-22-5 is a highly specific oxidoreductase enzyme that catalyzes the oxidative demethylation of sarcosine (N-methylglycine) to yield glycine, formaldehyde, and hydrogen peroxide. This precise catalytic activity makes it an indispensable reagent for enzymatic analysis and diagnostic applications. It is primarily utilized by manufacturers in the in-vitro diagnostic (IVD) industry, biochemical research laboratories, and enzymatic synthesis processes.

Application

• Clinical Diagnostics: A key component in enzymatic assay kits for the quantitative determination of creatinine in serum and urine, enabling accurate assessment of renal function.
• Biochemical Research: Used as a model enzyme in studies of flavoprotein oxidase mechanisms, substrate specificity, and enzyme kinetics.
• Enzymatic Reagent: Employed in coupled enzyme systems for the detection or quantification of other metabolites where sarcosine is an intermediate or byproduct.
• Food & Beverage Analysis: Potential application in analytical methods for detecting specific amino acids or related compounds in food matrices.
• Biocatalysis: Investigated for use in fine chemical synthesis pathways requiring selective N-demethylation reactions.

Basic Information

Item	Detail
Product Name	Sarcosine Oxidase
CAS No.	9029-22-5
Molecular Formula	N/A (Enzyme)
Molecular Weight	Approx. 42,000 Da (Monomer, species-dependent)
Synonyms	Sarcosine:oxygen oxidoreductase (demethylating); Sarcosine Oxidase (SOX); N-Methylglycine Oxidase; SOX Enzyme; EC 1.5.3.1; Sarcosine Oxidase from *Arthrobacter* sp.; Sarcosine Oxidase from *Corynebacterium* sp.; Sarcosine Oxidase from *Bacillus* sp.
EINECS	Contact for details

Quality Control

Our Sarcosine Oxidase is produced and tested under stringent quality management systems. Each lot undergoes comprehensive analysis to ensure high specific activity, purity, and functional performance suitable for diagnostic and research applications. A detailed Certificate of Analysis (COA) is provided with each shipment, documenting key parameters such as activity, protein content, and stability.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below. This product is hygroscopic (moisture-sensitive). Avoid repeated freeze-thaw cycles. Keep the container sealed under controlled, dry conditions to maintain optimal enzymatic activity.

Specification

Item	Specification
Appearance	Lyophilized powder or solution
Specific Activity	≥ 10 U/mg protein
Protein Content (Biuret)	Contact for details
Purity (SDS-PAGE)	≥ 90%
pH Optimum	~8.0
Temperature Optimum	~37°C
Stabilizers	May contain buffer salts (e.g., phosphate)
Solubility	Soluble in water or buffer

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.