Description

Cholesterol Oxidase is a highly specific enzyme that catalyzes the oxidation of cholesterol to 4-cholesten-3-one with the concomitant reduction of oxygen. This precise biochemical action makes it an indispensable reagent for the quantitative determination of cholesterol in clinical, food, and research settings. It is a critical component for manufacturers of diagnostic kits, research institutions, and quality control laboratories requiring accurate and reliable enzymatic analysis.

Application

• Clinical Diagnostics: Primary component in enzymatic colorimetric and electrochemical assays for measuring total and free cholesterol in human serum, plasma, and other biological fluids.
• Food & Beverage Analysis: Used for the quantification of cholesterol content in dairy products, meats, eggs, and other foodstuffs for nutritional labeling and quality control.
• Pharmaceutical Research: Employed in drug discovery and development studies targeting cholesterol metabolism, atherosclerosis, and related metabolic disorders.
• Biochemical Research: Essential tool for studying enzyme kinetics, cholesterol biosynthesis pathways, and membrane lipid dynamics in academic and industrial R&D.
• Industrial Biotechnology: Potential use in biosensor development for real-time monitoring of cholesterol in fermentation processes or environmental samples.

Basic Information

Product Name	Cholesterol Oxidase
CAS No.	9028-76-6
Molecular Formula	N/A (Enzyme)
Molecular Weight	Varies by source (typically ~30-60 kDa)
Synonyms	Cholesterol:oxygen oxidoreductase; 1.1.3.6; CHO; Cholesterol Oxidase (microbial); Sterol oxidase; COx
EINECS	Contact for details

Quality Control

Our Cholesterol Oxidase is produced and tested under a stringent quality management system. Each lot is subjected to rigorous analytical procedures to ensure high specific activity, purity, and consistency suitable for diagnostic and research applications. A comprehensive Certificate of Analysis (COA) detailing assay results, purity, and stability is provided with every shipment to guarantee traceability and compliance with your quality requirements.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store desiccated at -20°C. This product is hygroscopic (moisture-sensitive). Under these conditions, the product is stable for at least 24 months. For short-term use, it may be stored at 2-8°C for several weeks. Avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	Lyophilized powder
Specific Activity	≥ 20 U/mg protein
Protein Content (Biuret)	≥ 90%
Solubility	Soluble in water or buffer
pH Optimum	~7.0 - 7.5
Temperature Optimum	~37°C
Stability	Stable for 24 months at -20°C

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.