Description

3Alpha-Hydroxysteroid Dehydrogenase is a critical enzyme belonging to the oxidoreductase class, specifically catalyzing the NAD(P)+-dependent oxidation of 3α-hydroxysteroids. This product is essential for precise biochemical analysis and research into steroid hormone metabolism and regulation. It is primarily utilized by pharmaceutical R&D teams, diagnostic kit manufacturers, and academic research institutions focused on endocrinology and metabolic pathways.

Application

• Diagnostic Assay Development: A key component in enzymatic assays for the quantitative determination of specific steroid hormones (e.g., bile acids, androgens) in clinical and research settings.
• Pharmaceutical Research & Screening: Used in high-throughput screening (HTS) platforms to identify potential modulators or inhibitors of steroidogenic pathways for drug discovery.
• Biochemical Research: Fundamental tool for studying the kinetics, regulation, and genetic expression of 3α-hydroxysteroid dehydrogenase enzymes in various biological systems.
• Enzyme Engineering: Serves as a starting point for protein engineering studies aimed at improving stability, specificity, or catalytic efficiency for industrial biocatalysis.
• Quality Control in Steroid Production: Employed in enzymatic methods to assess the purity and concentration of steroid intermediates during manufacturing processes.

Basic Information

Product Name	3Alpha-Hydroxysteroid Dehydrogenase
CAS No.	9028-56-2
Molecular Formula	Protein (Enzyme)
Molecular Weight	Varies by source and subunit composition. Contact for details.
Synonyms	3α-HSD; 3α-Hydroxysteroid Dehydrogenase; 3-α-Hydroxysteroid:NAD(P)+ Oxidoreductase; EC 1.1.1.213; 3α-Hydroxysteroid Oxidoreductase; Testosterone Dehydrogenase; 3α-Hydroxysteroid (Nicotinamide Adenine Dinucleotide Phosphate) Dehydrogenase
EINECS	Contact for details.

Quality Control

Our 3Alpha-Hydroxysteroid Dehydrogenase is produced and tested under stringent quality management systems. Each lot undergoes comprehensive analysis to ensure high specific activity, purity, and functional stability. Certificates of Analysis (COA) detailing activity units/mg protein, purity by SDS-PAGE, and functional assay performance are available upon request. We adhere to relevant guidelines for research-grade biochemicals.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below. This material is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	Lyophilized powder or solution
Specific Activity	≥ [Value] units/mg protein
Purity (SDS-PAGE)	≥ 90%
Protein Content	By Bradford or similar method
Stabilizers/Additives	May contain buffer salts (e.g., phosphate, Tris) and stabilizers (e.g., glycerol, BSA)
Functional Activity	Confirmed by standard spectrophotometric assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.