Description

Ec 1.1.1.40 CAS NO 9028-47-1 is a high-purity enzyme preparation, specifically identified as Malate Dehydrogenase (MDH). This biocatalyst is essential for its role in the citric acid cycle (Krebs cycle), facilitating the reversible oxidation of malate to oxaloacetate. It is a critical reagent for research, diagnostic, and industrial applications requiring precise enzymatic activity, particularly in biochemical assays, metabolic pathway studies, and biosensor development.

Application

• Diagnostic Kits & Clinical Assays: A key component in enzymatic test kits for the determination of metabolites like malate, oxaloacetate, or NADH/NAD+ levels in clinical and research laboratories.
• Biochemical Research & Enzyme Studies: Used as a model enzyme for studying enzyme kinetics, inhibition, and the mechanisms of dehydrogenase activity in academic and pharmaceutical research.
• Metabolic Pathway Analysis: Essential for in vitro reconstitution and analysis of the citric acid cycle and related metabolic pathways in cell biology and systems biology.
• Biosensor Development: Incorporated into biosensing platforms for the detection of specific substrates or co-factors due to its high specificity and catalytic efficiency.
• Food & Beverage Analysis: Employed in enzymatic methods for quality control, such as assessing fruit juice authenticity or monitoring fermentation processes.
• Pharmaceutical Intermediate Synthesis: Potential use in biocatalysis for the stereoselective synthesis of chiral intermediates in fine chemical and active pharmaceutical ingredient (API) manufacturing.

Basic Information

Product Name	Ec 1.1.1.40 / Malate Dehydrogenase
CAS No.	9028-47-1
Molecular Formula	Protein (Enzyme)
Molecular Weight	~70 kDa (subunit dependent)
Synonyms	Malate Dehydrogenase; MDH; L-Malate:NAD+ Oxidoreductase; EC 1.1.1.40; Malic Dehydrogenase; NAD+-dependent Malate Dehydrogenase; Mitochondrial Malate Dehydrogenase; Cytosolic Malate Dehydrogenase; Dehydrogenase, Malate
EINECS	Contact for details

Quality Control

Our Malate Dehydrogenase is produced and tested under stringent quality management systems. Each lot is characterized for specific activity, purity, and stability to ensure reliable performance in sensitive applications. A comprehensive Certificate of Analysis (COA) detailing parameters such as activity units/mg protein, protein concentration, and impurity profiles is provided with every shipment to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a dry environment before opening to prevent condensation and moisture uptake. Avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	Clear to slightly opalescent solution or lyophilized powder
Identification (Activity)	Catalyzes the oxidation of L-Malate with NAD+
Specific Activity	≥ 800 units/mg protein (typical)
Protein Content (Biuret)	5 - 20 mg/mL (solution) or Contact for details (lyophilized)
Purity (SDS-PAGE)	≥ 90%
Stabilizers/Buffer	Contains glycerol and buffer salts (e.g., phosphate)
pH	6.5 - 7.5

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.