Description

Clostripain is a highly specific cysteine protease enzyme, isolated from *Clostridium histolyticum*, known for its unique ability to cleave peptide bonds at the carboxyl side of arginine residues. This specificity makes it an invaluable tool for precise protein sequencing, peptide mapping, and structural analysis in advanced biochemical research. It is essential for laboratories and production facilities in the biopharmaceutical, proteomics, and diagnostic reagent development sectors requiring high-purity enzymatic tools.

Application

• Protein Sequencing & Peptide Mapping: Used for the specific cleavage of arginyl bonds to generate predictable fragments for mass spectrometry analysis.
• Biopharmaceutical Research: Employed in the characterization of therapeutic proteins, antibodies, and recombinant products.
• Proteomics Studies: A key enzyme in bottom-up proteomics for digesting complex protein mixtures prior to LC-MS/MS analysis.
• Diagnostic Reagent Manufacturing: Incorporated into enzymatic assay kits and diagnostic systems requiring specific proteolytic activity.
• Structural Biology: Aids in the production of protein domains or fragments for X-ray crystallography and NMR studies.
• Cell Biology Research: Used in studies involving extracellular matrix proteins due to its action on collagen and other substrates.

Basic Information

Product Name	Clostripain
CAS No.	9028-00-6
Molecular Formula	N/A (Enzyme)
Molecular Weight	~50,000 Da (Approximate, varies by preparation)
Synonyms	Clostridiopeptidase B; Clostripain Protease; Arg-C Protease; Arg-C Endopeptidase; Clostridium Histolyticum Protease; Endoproteinase Arg-C; EC 3.4.22.8
EINECS	Contact for details

Quality Control

Our Clostripain is produced and tested under stringent quality management systems to ensure batch-to-batch consistency and high enzymatic activity. Each lot is characterized for specific activity, purity (via SDS-PAGE and HPLC), and absence of contaminating proteases. A comprehensive Certificate of Analysis (COA) detailing assay results, stability data, and storage conditions is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store lyophilized powder at -20°C. Reconstituted enzyme solutions should be aliquoted and stored at -20°C or below to minimize freeze-thaw cycles. The product is hygroscopic (moisture-sensitive); ensure containers are sealed tightly after each use in a low-humidity environment.

Specification

Item	Specification
Appearance	Lyophilized powder
Identification (Activity)	Cleaves synthetic substrate (e.g., BAEE) at Arg-X bonds
Specific Activity	≥ 40 units/mg protein
Purity (SDS-PAGE)	≥ 90% (Single band ~50 kDa under reducing conditions)
Protease Contamination	No detectable trypsin, chymotrypsin, or aminopeptidase activity
Protein Content (Biuret)	60-80%
Solubility	Soluble in water or buffer (50 mM Tris, 5 mM CaCl2, pH 7.6)
Storage Buffer (Lyophilized)	Tris-HCl, Calcium Acetate

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.