Description

Citrate Synthase CAS NO 9027-96-7 is a critical enzyme that catalyzes the first committed step of the citric acid cycle, a fundamental metabolic pathway. Its reliable performance is essential for research in cellular respiration, metabolic studies, and diagnostic assay development. This high-purity enzyme is a key reagent for biochemists, pharmaceutical researchers, and diagnostic manufacturers requiring consistent and specific activity for their applications.

Application

• Biochemical Research & Metabolic Pathway Analysis: Essential for in vitro studies of the Krebs cycle, mitochondrial function, and energy metabolism.
• Diagnostic Kit Manufacturing: Used as a key component in clinical diagnostic assays for measuring citrate, ATP, or Coenzyme A levels.
• Pharmaceutical Development: Employed in drug discovery screens targeting metabolic disorders and mitochondrial diseases.
• Enzyme Activity Assays & Standardization: Serves as a standard or coupling enzyme in spectrophotometric and fluorometric assays.
• Academic & Industrial Teaching: Used in educational laboratories to demonstrate enzyme kinetics and central metabolism principles.
• Food & Beverage Analysis: Applied in enzymatic methods for the quantification of citric acid and related metabolites in quality control.

Basic Information

Product Name	Citrate Synthase
CAS No.	9027-96-7
Molecular Formula	N/A (Enzyme)
Molecular Weight	~100,000 Da (varies by source)
Synonyms	Citrate (si)-synthase; Citrate condensing enzyme; Oxaloacetate transacetase; CS; Citrate oxaloacetate-lyase [(pro-3S)-CH2COO- → acetyl-CoA]; EC 4.1.3.7
EINECS	Contact for details

Quality Control

Our Citrate Synthase is produced and tested under stringent conditions to ensure high specific activity, purity, and lot-to-lot consistency suitable for research and diagnostic applications. Each batch is accompanied by a comprehensive Certificate of Analysis (COA) detailing specific activity, purity level (by SDS-PAGE), and stability data. We adhere to relevant quality management standards to support our global B2B clients.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below in a non-frost-free freezer. The product is hygroscopic (moisture-sensitive); allow the vial to equilibrate to room temperature before opening to prevent condensation. For short-term use, aliquoting is recommended to avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	Lyophilized powder or solution
Identification (Activity)	Conforms
Specific Activity	> 50 units/mg protein (Contact for specific grade)
Purity (SDS-PAGE)	> 90%
Protein Content (Biuret)	Provided on COA
Stabilizers / Buffer	Potassium phosphate, EDTA (typical)
Microbial Limits	Meets specification for biological products

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.