Description

5'-Nucleotidase From Crotalus Atrox Venom* is a highly purified enzyme derived from the venom of the Western diamondback rattlesnake, specifically catalyzing the hydrolysis of nucleotides. This product is critical for biochemical research and diagnostic applications where precise nucleotide metabolism analysis is required. It is primarily utilized by research institutions, pharmaceutical R&D departments, and in vitro diagnostic (IVD) kit manufacturers for enzymatic assays and metabolic pathway studies.

Application

• Biochemical Research: Used as a key reagent in enzymatic studies of nucleotide metabolism, purine salvage pathways, and signal transduction mechanisms.
• Diagnostic Assays: Employed in the development of clinical diagnostic kits for measuring 5'-nucleotidase activity in serum, aiding in the diagnosis of hepatobiliary diseases.
• Pharmaceutical Development: Serves as a tool enzyme for screening potential enzyme inhibitors or modulators in drug discovery pipelines targeting nucleotide-related disorders.
• Cell Biology Studies: Applied in research to investigate ecto-5'-nucleotidase (CD73) activity and its role in immunology, cancer biology, and inflammation.
• Enzyme Characterization: Utilized as a standard or reference material for the characterization and validation of other nucleotidase enzymes.
• Food & Beverage Analysis: Potential use in enzymatic methods for the analysis of nucleotide content in food products and supplements.

Basic Information

Product Name	5'-Nucleotidase From Crotalus Atrox Venom*
CAS No.	9027-73-0
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	5'-Ribonucleotide Phosphohydrolase; Ecto-5'-Nucleotidase (snake venom); CD73 (Crotalus atrox); 5'-NT; EC 3.1.3.5; Nucleotide Phosphatase; 5'-AMP Phosphatase
EINECS	Contact for details

Quality Control

Our 5'-Nucleotidase From Crotalus Atrox Venom* is produced under stringent quality management systems. Each lot undergoes rigorous analytical testing to ensure high specific activity, purity, and consistency. Certificates of Analysis (COA) detailing assay results, purity profiles, and stability data are provided with every shipment to guarantee compliance with your research and manufacturing standards.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C. This material is hygroscopic (moisture-sensitive); ensure the container is kept sealed in a dry environment to maintain stability and enzymatic activity. For long-term storage, consider aliquoting and freezing at -20°C or below.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (IR)	Conforms to standard
Specific Activity	≥ 50 units/mg protein
Protein Content (Biuret)	≥ 90%
Purity (SDS-PAGE)	Single major band
pH (in solution)	6.0 - 7.5
Loss on Drying	≤ 5.0 %
Endotoxin (LAL)	< 1.0 EU/mg

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.