Description

Acetate Kinase is a critical enzyme that catalyzes the reversible transfer of a phosphate group from ATP to acetate, forming acetyl phosphate and ADP. This reaction is fundamental to metabolic pathways involving acetate activation and energy transfer, making it an indispensable tool for biochemical research and industrial biocatalysis. It is essential for scientists and manufacturers in the pharmaceutical, biotechnology, and diagnostic sectors who require high-purity enzymes for metabolic studies, enzymatic assays, and the development of biosynthetic processes.

Application

• Biochemical Research & Metabolic Pathway Analysis: Used as a key reagent in enzymatic studies to investigate acetate metabolism, energy charge, and phosphate transfer mechanisms in vitro.
• Diagnostic Kit Manufacturing: Incorporated into enzymatic assay kits for the clinical and research-based quantification of ATP, ADP, or acetate.
• Pharmaceutical Development: Employed in biocatalytic processes for the synthesis of fine chemicals and active pharmaceutical intermediates (APIs) that utilize acetyl phosphate as an activated substrate.
• Enzyme Cascade Reactions: Serves as a component in multi-enzyme systems for cofactor regeneration or the driven synthesis of target molecules in synthetic biology applications.
• Academic & Industrial Enzymology: Utilized for teaching and research purposes to demonstrate kinase activity, enzyme kinetics, and inhibitor screening.
• Food & Beverage Analysis: Applied in enzymatic methods for the detection and quantification of acetic acid or related compounds in quality control laboratories.

Basic Information

Product Name	Acetate Kinase
CAS No.	9027-42-3
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Acetate Kinase (AK); ATP:acetate phosphotransferase; Acetokinase; Acetate kinase (phosphorylating); Acetate kinase, E.C. 2.7.2.1; AK enzyme; Acetyl phosphate synthetase
EINECS	Contact for details

Quality Control

Our Acetate Kinase is produced and tested under stringent quality management systems. Each lot undergoes rigorous analytical testing to ensure high specific activity, purity, and consistency. A comprehensive Certificate of Analysis (COA) is provided with each shipment, detailing parameters such as activity units, protein content, and purity assays. We support compliance with relevant ISO and GMP guidelines for research and manufacturing use.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below. This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent moisture absorption, which can affect enzymatic activity. Avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder or solution
Identification (Activity)	Conforms
Specific Activity	≥ [Value] units/mg protein
Purity (SDS-PAGE)	≥ 90%
Protein Content (Bradford)	Reported on COA
pH (in solution)	7.0 - 7.5
Endotoxin Level	< 1.0 EU/μg (for specific grades)
Storage Buffer	Reported on COA

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.