Description

Crotonase is a critical enzyme that catalyzes the reversible hydration of crotonyl-CoA to 3-hydroxybutyryl-CoA, a fundamental step in fatty acid metabolism and ketone body utilization. Its high specificity and catalytic efficiency make it an indispensable tool for biochemical research and industrial biocatalysis. This product is essential for researchers in metabolic pathway analysis, enzymology studies, and for companies developing biosynthetic routes for fine chemicals and pharmaceuticals.

Application

• Biochemical Research: Used as a key reagent for studying the β-oxidation pathway of fatty acids and ketone body metabolism in vitro.
• Enzyme Kinetics & Mechanism Studies: Employed to investigate enzyme catalysis, inhibition, and structure-function relationships due to its well-characterized reaction.
• Diagnostic Kit Manufacturing: Serves as a component in enzymatic assay kits designed to measure metabolites related to fatty acid oxidation disorders.
• Biocatalysis & Synthetic Biology: Utilized in engineered metabolic pathways for the production of chiral intermediates, biofuels, and specialty chemicals.
• Pharmaceutical Development: Applied in research targeting metabolic diseases, where modulation of ketogenesis or fatty acid metabolism is therapeutic.
• Educational Purposes: Used in university laboratories for teaching advanced concepts in enzymology and intermediary metabolism.

Basic Information

Product Name	Crotonase
CAS No.	9027-13-8
Molecular Formula	N/A (Enzyme)
Molecular Weight	~160 kDa (Tetramer) - Contact for details
Synonyms	Enoyl-CoA Hydratase; 3-Hydroxyacyl-CoA Dehydratase; Short-chain Enoyl-CoA Hydratase; EC 4.2.1.17; Crotonoyl-CoA Hydratase; Mitochondrial Enoyl-CoA Hydratase; ECHS1
EINECS	Contact for details

Quality Control

Our Crotonase is produced and tested under stringent conditions to ensure high activity, purity, and batch-to-batch consistency. Each lot is characterized for specific activity and undergoes purity analysis. We provide full traceability and support our products with comprehensive documentation. Certificates of Analysis (COA) detailing activity units/mg protein, purity, and stability data are available upon request.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below in a non-frost-free freezer. The product is hygroscopic (moisture-sensitive). Avoid repeated freeze-thaw cycles. Aliquot upon receipt for optimal preservation of enzymatic activity.

Specification

Item	Specification
Appearance	Lyophilized powder or solution
Specific Activity	≥ [Value] units/mg protein
Purity (SDS-PAGE)	≥ 95%
Protein Content (Bradford)	Contact for details
Stabilizers/Additives	May contain buffer salts (e.g., Tris, phosphate) and glycerol
Solubility	Soluble in appropriate aqueous buffers
Biological Source	Recombinant E. coli or bovine liver

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.