Description

Poly(A) Polymerase is a highly specific enzyme that catalyzes the template-independent addition of adenosine monophosphate residues to the 3'-hydroxyl termini of RNA molecules. This unique activity is critical for modifying RNA structure and function, enabling key downstream analytical and synthetic processes. It is an essential reagent for molecular biology research, pharmaceutical development, and diagnostic applications requiring precise RNA tailing.

Application

• Poly(A) Tailing of RNA: Essential for preparing mRNA for cDNA synthesis and next-generation sequencing (NGS) library construction.
• 3'-End Labeling of RNA: Used to incorporate radioactive or fluorescent nucleotides for detection and analysis in blotting assays.
• mRNA Stability and Translation Studies: Employed to investigate the role of poly(A) tail length in regulating mRNA half-life and translational efficiency.
• Diagnostic Assay Development: A key component in kits for detecting and quantifying specific RNA targets, including viral RNAs.
• Synthetic Biology: Used in the enzymatic synthesis and modification of RNA constructs for therapeutic and research purposes.
• Microarray Analysis: Facilitates the amplification and labeling of RNA samples for gene expression profiling.

Basic Information

Product Name	Poly(A) Polymerase
CAS No.	9026-30-6
Molecular Formula	N/A (Enzyme)
Molecular Weight	~50-70 kDa (Source dependent)
Synonyms	Polyadenylate Polymerase; Poly(A) Polymerase, E. coli; Polynucleotide Adenylyltransferase; PAP; EC 2.7.7.19; RNA Adenylating Enzyme; ATP:RNA Adenylyltransferase
EINECS	Contact for details

Quality Control

Our Poly(A) Polymerase is produced under stringent quality management systems. Each lot undergoes rigorous functional activity assays, purity analysis, and testing for contaminating nucleases to ensure optimal performance in sensitive applications. A comprehensive Certificate of Analysis (COA) detailing specific activity, purity, and stability is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C in a non-frost-free freezer. The enzyme is supplied in a buffered glycerol solution. Avoid repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive).

Specification

Item	Specification
Appearance	Clear, colorless solution
Identification (Activity)	Functional activity confirmed by poly(A) tailing assay
Specific Activity	≥ 5,000 units/mg protein
Purity (SDS-PAGE)	≥ 95%
Contaminating RNase Activity	Not detectable
Contaminating DNase Activity	Not detectable
Endotoxin Level	< 0.1 EU/µg (for molecular biology grade)
Storage Buffer	50 mM Tris-HCl, 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, pH 7.9

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.