Description

Glyoxalase Ii From Bovine Liver is a purified enzyme preparation derived from bovine liver tissue, specifically the enzyme glyoxalase II (also known as hydroxyacylglutathione hydrolase). This biocatalyst plays a critical role in the glyoxalase system, which is essential for the detoxification of methylglyoxal and other reactive α-ketoaldehydes, protecting cellular components from damage. It is a vital reagent for biochemical research, enzymatic studies, and diagnostic kit development, serving scientists and manufacturers in the pharmaceutical, life science, and diagnostic industries who require high-purity, reliable enzymatic activity for their metabolic pathway investigations.

Application

• Biochemical Research: Used as a key enzyme in studies of the glyoxalase pathway, methylglyoxal metabolism, and cellular detoxification mechanisms.
• Enzymatic Assay Development: Serves as a critical component in the development of colorimetric, fluorometric, or spectrophotometric assays to measure glyoxalase II activity or related metabolites.
• Diagnostic Manufacturing: Incorporated into diagnostic kits and reagents for clinical or research applications targeting metabolic disorders or oxidative stress markers.
• Metabolic Pathway Analysis: Utilized in in vitro systems to elucidate the kinetics and regulation of the glyoxalase system and its connection to diabetes, aging, and neurological conditions.
• Pharmaceutical Screening: Employed in high-throughput screening platforms to identify potential inhibitors or activators of glyoxalase II for therapeutic drug discovery.
• Reference Standard: Acts as a purified standard for activity calibration and quality control in laboratories performing related enzymatic analyses.

Basic Information

Product Name	Glyoxalase Ii From Bovine Liver
CAS No.	9025-90-5
Molecular Formula	Protein (Enzyme)
Molecular Weight	Contact for details
Synonyms	Glyoxalase II; Hydroxyacylglutathione Hydrolase; S-2-Hydroxyacylglutathione Hydrolase; Glx II; EC 3.1.2.6; Methylglyoxalase II; Lactoylglutathione Lyase (deprecated); Bovine Liver Glyoxalase II
EINECS	Contact for details

Quality Control

Our Glyoxalase Ii From Bovine Liver is produced and tested under stringent conditions to ensure high specific activity, purity, and batch-to-batch consistency suitable for research and industrial applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing parameters such as specific activity, protein content, and purity profile. We adhere to relevant quality management principles to support our customers' needs for reliable and traceable biochemical reagents.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below. This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent moisture absorption, which can affect activity. Avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	Lyophilized powder or solution
Specific Activity	≥ [Value] units/mg protein
Protein Content (Biuret)	Contact for details
Purity (SDS-PAGE)	≥ 90%
pH (in solution)	6.0 - 8.0
Solubility	Soluble in water or specified buffer
Stability	Stable under recommended storage conditions

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.