Description

Ec 3.1.4.2 is a high-purity enzyme preparation, specifically a phospholipase C, identified by the CAS number 9025-85-8. This enzyme is critical for catalyzing the hydrolysis of phospholipids, a fundamental reaction in lipid metabolism and cell signaling research. It is an essential reagent for biochemists and process engineers in the pharmaceutical, biotechnology, and diagnostic industries, where precise enzymatic activity is required for research, development, and production.

Application

• Biochemical Research: Used in studies of cell membrane structure, signal transduction pathways, and lipid second messengers like diacylglycerol (DAG) and inositol phosphates.
• Pharmaceutical Development: Employed in the synthesis of specific phospholipid derivatives and as a tool enzyme in drug discovery platforms targeting lipid-related diseases.
• Diagnostic Reagent Manufacturing: A key component in enzymatic assay kits designed to measure phospholipid levels or related enzymatic activities in clinical samples.
• Food Science & Technology: Applied in the modification of phospholipids (e.g., lecithin) to alter emulsification properties or create specific functional ingredients.
• Industrial Biotechnology: Potential use in biocatalytic processes for the targeted breakdown or synthesis of phospholipids in controlled environments.

Basic Information

Product Name	Ec 3.1.4.2
CAS No.	9025-85-8
Molecular Formula	Protein (Enzyme)
Molecular Weight	Varies by source and isoform
Synonyms	Phospholipase C; PLC; 1-Phosphatidylinositol Phosphodiesterase; Phosphatidylinositol Phosphodiesterase; Clostridium perfringens Alpha Toxin (PLC activity); Lecithinase C; Phosphatidylcholine Cholinephosphohydrolase; PI-PLC
EINECS	Contact for details

Quality Control

Our Ec 3.1.4.2 is produced and tested under stringent quality management systems. Each batch is characterized for specific activity, purity (via SDS-PAGE), and absence of contaminating enzymatic activities. Certificates of Analysis (COA) detailing activity units/mg protein, pH optimum, stability data, and storage buffer composition are provided to ensure reproducible performance in your applications.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store frozen at -20°C or below. Avoid repeated freeze-thaw cycles. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	Lyophilized powder or solution
Identification (Activity)	Conforms (Hydrolyzes phosphatidylinositol)
Specific Activity	> X units/mg protein (Grade dependent)
Purity (SDS-PAGE)	> 90%
Protein Content (Bradford)	Reported on COA
pH Optimum	~7.0 - 7.5 (Varies by source)
Stabilizers/Buffer	Reported on COA

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.