Description

3'-Nucleotidase is a hydrolytic enzyme that specifically catalyzes the dephosphorylation of 3'-nucleotides, releasing inorganic phosphate and the corresponding nucleoside. This precise catalytic activity makes it an indispensable tool for biochemical research and diagnostic applications, particularly in the study of nucleotide metabolism and enzyme kinetics. It is essential for researchers and manufacturers in the life sciences, pharmaceutical development, and in vitro diagnostic (IVD) kit production who require high-purity, reliable enzymatic reagents.

Application

• Biochemical Research: Used as a key reagent in enzymatic assays to study nucleotide metabolism, phosphatase activity, and signal transduction pathways.
• Pharmaceutical Development: Employed in the research and quality control of nucleotide-based drugs, including antivirals and anticancer agents.
• Diagnostic Kit Manufacturing: A critical component in the formulation of clinical diagnostic kits for measuring specific enzyme activities or nucleotide levels in biological samples.
• Enzyme Kinetics Studies: Serves as a model enzyme for investigating enzyme mechanisms, inhibition, and catalytic efficiency due to its specific substrate profile.
• Molecular Biology: Utilized in specific protocols for nucleic acid manipulation and analysis where controlled removal of 3'-phosphate groups is required.

Basic Information

Product Name	3'-Nucleotidase
CAS No.	9025-84-7
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	3'-Ribonucleotide Phosphatase; 3'-RNase; Uridine 3'-Nucleotidase; 3'-Mononucleotidase; EC 3.1.3.6; 3'-Phosphatase; Nucleotide 3'-Phosphatase
EINECS	Contact for details

Quality Control

Our 3'-Nucleotidase is produced and tested under stringent quality management systems to ensure batch-to-batch consistency and high enzymatic activity. Each lot is characterized for specific activity, purity, and absence of interfering contaminants. A comprehensive Certificate of Analysis (COA) detailing assay results, purity profiles, and stability data is provided with every shipment to support your quality and regulatory requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C. This product is hygroscopic (moisture-sensitive) and should be handled in a dry environment to maintain stability and activity. For long-term storage, desiccants are recommended.

Specification

Item	Specification
Appearance	Lyophilized powder or solution
Specific Activity	≥ XX units/mg protein
Purity (SDS-PAGE)	≥ 90%
Protein Content (Biuret)	XX-XX mg/vial
pH (in solution)	7.0 - 7.5
Identification (IR / Activity)	Conforms
Endotoxin (LAL)	< 1.0 EU/mg

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.