Description

Phosphodiesterase I is a crucial hydrolytic enzyme that catalyzes the cleavage of phosphodiester bonds in nucleic acids. This specificity makes it an indispensable tool for molecular biology research, diagnostics, and biochemical analysis. It is primarily utilized by research institutions, pharmaceutical developers, and diagnostic kit manufacturers for applications such as nucleotide sequencing, DNA/RNA modification studies, and enzyme activity assays.

Application

• Nucleic Acid Research & Sequencing: Used for the stepwise degradation of oligonucleotides from the 3'-end to aid in sequencing and structural analysis.
• Biochemical Assay Development: Serves as a key component in enzymatic assay kits for studying other phosphodiesterases or nucleic acid-interacting proteins.
• Diagnostic Manufacturing: Incorporated into diagnostic reagents and kits for clinical testing and genetic analysis.
• Pharmaceutical R&D: Employed in drug discovery research targeting nucleotide metabolism and related pathways.
• Enzyme Characterization: Acts as a standard or reference enzyme in comparative biochemical studies.
• Biotechnology Processes: Used in controlled nucleic acid digestion steps during the production of fine biochemicals.

Basic Information

Product Name	Phosphodiesterase I
CAS No.	9025-82-5
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	5'-Exonuclease; Snake Venom Exonuclease; SVPDE; EC 3.1.4.1; Exodeoxyribonuclease I; Oligonucleate 5'-nucleotidohydrolase; PDE I; Venom Phosphodiesterase
EINECS	Contact for details

Quality Control

Our Phosphodiesterase I is produced and tested under stringent quality management protocols to ensure high purity, specific activity, and batch-to-batch consistency. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing key parameters such as enzymatic activity, protein content, and purity. We adhere to relevant quality standards suitable for research and in-vitro diagnostic use.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	Lyophilized powder or solution
Identification (Activity)	Conforms
Specific Activity	> 100 units/mg protein
Purity (SDS-PAGE)	> 90%
Protein Content (Biuret)	Reported per COA
pH (in solution)	6.0 - 8.0
Endotoxin (LAL)	< 1.0 EU/μg

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.