Description

Mung Bean Nuclease is a highly specific single-strand endonuclease derived from mung bean sprouts, widely recognized for its precision in molecular biology applications. Its core value lies in its ability to selectively degrade single-stranded DNA and RNA while leaving double-stranded nucleic acids intact, making it an indispensable tool for genetic analysis and manipulation. This enzyme is essential for researchers and technicians in the biotechnology, pharmaceutical, and diagnostic industries, particularly for applications requiring the removal of single-stranded overhangs, mapping mutations, and analyzing nucleic acid structure.

Application

• Removal of single-stranded overhangs from DNA fragments to create blunt ends for cloning.
• Mapping mutations and polymorphisms via S1 nuclease mapping techniques.
• Analysis of nucleic acid secondary structure and DNA/RNA hybridization.
• Cleavage of hairpin loops formed during cDNA synthesis.
• Specific digestion of single-stranded regions in PCR products or recombinant DNA constructs.
• Quality control in oligonucleotide synthesis and next-generation sequencing (NGS) library preparation.
• Research in molecular genetics, virology, and gene expression studies.

Basic Information

Product Name	Mung Bean Nuclease
CAS No.	9025-65-4
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Mung Bean Nuclease S1; Nuclease S1 (Aspergillus oryzae); Single-Strand-Specific Nuclease; EC 3.1.30.1; Deoxyribonuclease S1; Ribonuclease S1; Endonuclease S1; Single-Stranded Nucleic Acid Endonuclease
EINECS	Contact for details

Quality Control

Our Mung Bean Nuclease is produced and tested under stringent quality management systems to ensure batch-to-batch consistency and high enzymatic activity. Each lot is subjected to rigorous analytical testing, including activity assays, purity assessment, and tests for contaminating nucleases. A comprehensive Certificate of Analysis (COA) detailing purity, specific activity, and stability is provided with every shipment to support your quality assurance and regulatory compliance needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below for long-term stability. The product is hygroscopic (moisture-sensitive). Aliquot to avoid repeated freeze-thaw cycles and keep desiccated.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder or solution
Identification (Activity)	Conforms
Specific Activity	≥ 100,000 units/mg protein
Purity (SDS-PAGE)	≥ 90%
Double-Stranded DNA Degradation	≤ 0.1%
RNase Contamination	Not detected
Protease Contamination	Not detected
pH (in storage buffer)	5.0 - 6.0
Endotoxin	< 0.1 EU/µg (if applicable)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.