Description

Deoxyribonuclease Ii is a hydrolytic enzyme that specifically cleaves phosphodiester bonds in DNA, producing 3'-phosphomononucleotides and oligonucleotides. This specificity makes it an indispensable tool for nucleic acid research and various biotechnological processes. It is primarily utilized by research institutions, diagnostic kit manufacturers, and biopharmaceutical companies for applications ranging from molecular biology to the removal of contaminating DNA from protein preparations.

Application

• Molecular Biology Research: Used in DNA footprinting, mapping, and sequencing studies due to its specific cleavage pattern.
• Biopharmaceutical Production: Critical for the removal of host cell DNA during downstream purification of therapeutic proteins and vaccines to ensure product safety and purity.
• Diagnostic Kit Manufacturing: Employed as a key reagent in kits designed for DNA sample preparation and analysis.
• Cell Culture & Tissue Engineering: Applied to digest DNA released from lysed cells, reducing viscosity and improving the yield and quality of cell-derived products.
• Forensic Science: Aids in the processing of complex DNA samples by selective digestion of certain DNA structures.
• Food Safety Testing: Used in enzymatic preparation steps for the detection of pathogenic bacterial DNA in food samples.

Basic Information

Product Name	Deoxyribonuclease Ii
CAS No.	9025-64-3
Molecular Formula	N/A (Enzyme)
Molecular Weight	Approx. 38 kDa (monomer)
Synonyms	DNase II; Acid Deoxyribonuclease; Deoxyribonuclease II; Deoxyribonucleate 3'-nucleotidohydrolase; Acid DNase; Spleen DNase; EC 3.1.22.1
EINECS	Contact for details

Quality Control

Our Deoxyribonuclease Ii is produced and tested under a stringent quality management system. Each batch is characterized for specific activity, purity, and the absence of contaminating RNase and protease activities. Certificates of Analysis (COA) detailing specifications such as activity units/mg, protein content, and pH optimum are provided to ensure it meets the rigorous standards required for research and industrial applications.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store lyophilized powder at 2-8°C. Reconstituted enzyme solutions should be aliquoted and stored at -20°C or below to prevent loss of activity. The product is hygroscopic (moisture-sensitive).

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (Activity)	Conforms
Specific Activity	≥ 2,000 units/mg protein
Protein Content (Biuret)	≥ 80%
pH Optimum	4.5 - 5.5
RNase Activity	Not detectable
Protease Activity	Not detectable
Solubility	Clear, colorless solution in buffer

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.