Description

Ec 3.3.1.1 CAS NO 9025-54-1 is a high-purity enzyme preparation, specifically a carboxylesterase, essential for catalyzing the hydrolysis of ester bonds in a wide range of substrates. This biocatalyst is critical for ensuring precise and efficient reactions in research and industrial synthesis, where specificity and activity are paramount. It is primarily utilized by the pharmaceutical, biotechnology, and fine chemical industries for applications including biocatalysis, metabolic studies, and the synthesis of chiral intermediates.

Application

• Biocatalysis for Chiral Synthesis: Key for the enantioselective hydrolysis of esters to produce single-enantiomer intermediates for active pharmaceutical ingredients (APIs).
• Pharmaceutical Research & Development: Used in metabolic pathway studies and prodrug activation mechanisms.
• Diagnostic Reagent Formulation: Incorporated into enzymatic assay kits for clinical and research diagnostics.
• Fine Chemical Production: Employed in the synthesis of specialty chemicals, flavors, and fragrances through ester modification.
• Biotechnology Processing: Applied in bioprocessing for the modification of biomolecules and in biosensor development.
• Academic & Industrial Research: Serves as a standard enzyme for biochemical research, enzyme kinetics studies, and method development.

Basic Information

Product Name	Ec 3.3.1.1
CAS No.	9025-54-1
Molecular Formula	N/A (Enzyme Preparation)
Molecular Weight	Varies by isoform
Synonyms	Carboxylesterase; Carboxylic Ester Hydrolase; EC 3.1.1.1; B-Esterase; Liver Esterase; Aryl-ester Hydrolase; Esterase from porcine liver; Carboxylate Esterase
EINECS	Contact for details

Quality Control

Our enzyme preparations are produced and tested under strict quality management systems to ensure batch-to-batch consistency and high specific activity. Each lot is characterized for purity, activity, and stability. Certificates of Analysis (COA) detailing assay results, protein content, and specific activity are provided and available upon request to support your quality and regulatory requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C. This product is hygroscopic (moisture-sensitive). For long-term stability, avoid repeated freeze-thaw cycles and consider storage at -20°C.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (IR)	Conforms to standard
Protein Content (Biuret)	≥ 90%
Specific Activity	≥ 50 units/mg protein
Loss on Drying	≤ 5.0 %
pH (in solution)	6.0 - 8.0
Heavy Metals (as Pb)	≤ 10 ppm
Microbial Limits	Meets standard for enzyme preparations

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.