Description

Alpha,α-Trehalose Glucohydrolase is a highly specific enzyme that catalyzes the hydrolysis of the non-reducing disaccharide trehalose into two glucose molecules. This precise biocatalytic activity is critical for controlling sugar metabolism and osmoregulation in various biological and industrial processes. It is an essential reagent for research in biochemistry and microbiology, as well as for applications in the food, pharmaceutical, and diagnostic industries where the manipulation of trehalose is required.

Application

• Biochemical Research: Used as a key tool enzyme for studying carbohydrate metabolism, enzyme kinetics, and metabolic pathways in microorganisms, plants, and insects.
• Diagnostic Kit Manufacturing: Employed in the production of clinical diagnostic reagents for the specific detection and quantification of trehalose or related metabolic markers.
• Food Science & Analysis: Applied in food laboratories for the enzymatic determination of trehalose content in various food products, including mushrooms, yeast extracts, and processed foods.
• Pharmaceutical Development: Utilized in R&D for drug discovery programs targeting trehalose metabolism, particularly in relation to mycobacterial (e.g., tuberculosis) and fungal infections.
• Industrial Biotechnology: Serves as a model enzyme or a processing aid in biotechnological applications involving trehalose breakdown, such as in biofuel production or specialty chemical synthesis.

Basic Information

Product Name	Alpha,α-Trehalose Glucohydrolase
CAS No.	9025-52-9
Molecular Formula	N/A (Enzyme)
Molecular Weight	Approx. 160,000 Da (varies by source)
Synonyms	Trehalase; α,α-Trehalase; α,α-Trehalose Glucohydrolase; Trehalose 1-Glucohydrolase; α,α-Trehalose-1-C-D-Glucohydrolase; Mycodextranase; Trehalase Enzyme; EC 3.2.1.28
EINECS	Contact for details

Quality Control

Our enzyme products are manufactured and tested under stringent quality management systems. Each batch of Alpha,α-Trehalose Glucohydrolase is characterized for specific activity, purity, and stability to ensure consistent performance. Certificates of Analysis (COA) detailing key parameters such as activity units, protein content, and purity are provided with every shipment to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at 2-8°C. This product is hygroscopic (moisture-sensitive); ensure the container is kept sealed in a dry environment to prevent loss of activity.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (Activity)	Catalyzes the hydrolysis of trehalose to glucose
Specific Activity	> 10 units/mg protein
Protein Content (Biuret)	> 90%
pH Optimum	5.0 - 6.5
Temperature Optimum	40 - 50°C
Solubility	Soluble in water or buffer

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.