Description

Nuclease From Penicillium Citrinum is a highly specific enzyme that catalyzes the hydrolysis of nucleic acids, primarily targeting RNA. This product is valued for its ability to effectively remove RNA contamination from protein preparations and other biological samples, ensuring high-purity downstream results. It is an essential reagent for molecular biology research, pharmaceutical manufacturing, and diagnostic kit production where the elimination of nucleic acids is critical.

Application

• RNA Removal in Protein Purification: Critical for eliminating contaminating RNA from protein extracts, viral vaccines, and therapeutic protein preparations.
• Molecular Biology Research: Used in nucleic acid extraction protocols to digest unwanted RNA, improving the purity of DNA samples for PCR, sequencing, and cloning.
• Pharmaceutical Manufacturing: Employed in the production of biologics to ensure final products are free from nucleic acid impurities, meeting stringent regulatory standards.
• Diagnostic Reagent Production: A key component in the formulation of diagnostic kits and reagents where specific nuclease activity is required.
• Cell Culture & Virology: Used to treat fetal bovine serum (FBS) and other cell culture media to inactivate viral RNA and nucleases.
• Food & Beverage Analysis: Applied in analytical laboratories for sample preparation to hydrolyze RNA prior to analysis.

Basic Information

Product Name	Nuclease From Penicillium Citrinum
CAS No.	9025-44-9
Molecular Formula	N/A (Enzyme)
Molecular Weight	N/A (Enzyme)
Synonyms	Penicillium Citrinum Nuclease; P1 Nuclease; Nuclease P1; Single-Strand-Specific Nuclease; Endonuclease P1; EC 3.1.30.1; Polynucleotidase; Aspergillus Nuclease P1 (common misnomer)
EINECS	Contact for details

Quality Control

Our Nuclease From Penicillium Citrinum is produced under strict quality management systems. Each batch undergoes rigorous testing for specific activity, purity (via SDS-PAGE and HPLC), and the absence of contaminating protease and phosphatase activities. Certificates of Analysis (COA) detailing assay results, stability, and microbiological data are available upon request to ensure it meets the requirements for research and industrial applications.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C. This material is hygroscopic (moisture-sensitive); keep the container tightly sealed in a dry environment. Avoid repeated freeze-thaw cycles to maintain optimal enzymatic activity.

Specification

Item	Specification
Appearance	Lyophilized powder or solution
Identification (IR)	Conforms
Specific Activity	> 20,000 U/mg protein
Protein Content (Biuret)	> 90%
pH (in solution)	5.0 - 7.0
Protease Activity	Not detectable
Phosphatase Activity	Not detectable
Endotoxin (LAL)	< 1.0 EU/mg

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.