Description

α-Mannosidase is a hydrolytic enzyme that specifically cleaves terminal, non-reducing α-D-mannose residues from glycoproteins, glycolipids, and oligosaccharides. This precise catalytic activity is crucial for the structural analysis and modification of complex carbohydrates in research and industrial biocatalysis. It is an essential tool for biochemists, glycobiologists, and manufacturers in the pharmaceutical, diagnostic, and biotechnology sectors requiring high-purity enzymatic reagents.

Application

• Glycoprotein Structural Analysis: Used to sequentially remove mannose residues from N-linked glycans to determine branching patterns and structure.
• Biopharmaceutical Characterization: Critical for analyzing the glycosylation profile of monoclonal antibodies and other therapeutic proteins to ensure batch consistency and efficacy.
• Diagnostic Kit Manufacturing: Employed as a key component in enzymatic assay kits for the detection of specific lysosomal storage disorders, such as α-mannosidosis.
• Biocatalysis & Synthesis: Facilitates the synthesis of specific oligosaccharide structures or the modification of glycoconjugates for research and development.
• Food & Feed Research: Applied in studies to modify plant-based polysaccharides or to develop enzymes for improving nutritional value.
• Quality Control in Sugar Processing: Used to assay and control the composition of mannose-containing polysaccharide products.

Basic Information

Product Name	α-Mannosidase
CAS No.	9025-42-7
Molecular Formula	N/A (Enzyme)
Molecular Weight	Varies by source and purification (typically ~110 kDa for jack bean)
Synonyms	α-Mannosidase; α-D-Mannoside Mannohydrolase; Exo-α-1,2-1,3-1,6-Mannosidase; Jack Bean α-Mannosidase; Lysosomal α-Mannosidase; MAN2B1; MANBA
EINECS	232-803-9

Quality Control

Our α-Mannosidase is produced and tested under stringent quality management systems. Each lot is characterized for specific activity, purity, and absence of contaminating glycosidase activities to ensure reliable and reproducible performance. A comprehensive Certificate of Analysis (COA) detailing specifications such as activity (U/mg), protein content, and stability is provided with every shipment to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake. For the supplied buffer or glycerol solutions, store at 2-8°C.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder or clear solution
Identification (Activity)	Catalyzes hydrolysis of p-nitrophenyl-α-D-mannopyranoside
Specific Activity	> 50 U/mg protein (Typical, varies by grade)
Purity (SDS-PAGE)	≥ 90% (Single major band)
Contaminating Activities	Free from β-Mannosidase, β-N-Acetylglucosaminidase, other exoglycosidases
Protein Content (Biuret)	Reported on COA
pH Optimum	4.0 - 4.5 (Acetate buffer)
Stability	≥ 12 months at recommended storage temperature

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.