Description

Carboxypeptidase B CAS NO 9025-24-5 is a highly specific proteolytic enzyme that catalyzes the release of C-terminal lysine and arginine residues from peptides and proteins. This precision is critical for ensuring product quality and process control in advanced biomanufacturing and research applications. It is an essential reagent for biopharmaceutical companies, diagnostic kit manufacturers, and research institutions focused on protein sequencing, peptide mapping, and recombinant protein processing.

Application

• Recombinant Protein & Monoclonal Antibody (mAb) Purification: Removal of C-terminal lysine residues from therapeutic antibodies to ensure charge homogeneity and batch consistency.
• Peptide Mapping & Protein Sequencing: Used in analytical laboratories for structural characterization and confirmation of amino acid sequences.
• Insulin Production: Critical in the enzymatic conversion of proinsulin to active insulin.
• Diagnostic Reagent Manufacturing: A key component in enzymatic assay kits for clinical diagnostics and life science research.
• Biopharmaceutical Process Development: Employed in downstream processing to cleave affinity tags from fusion proteins.
• Food Science Research: Used in studies of protein hydrolysis and flavor development.

Basic Information

Product Name	Carboxypeptidase B
CAS No.	9025-24-5
Molecular Formula	N/A (Enzyme from bovine or porcine pancreas)
Molecular Weight	~34 kDa (Monomer)
Synonyms	Carboxypeptidase B; CPB; Protaminase; Peptidyl-L-lysine(-L-arginine) hydrolase; Carboxypolypeptidase; 3.4.17.2; Pancreatic carboxypeptidase B
EINECS	232-807-2

Quality Control

Our Carboxypeptidase B is manufactured and tested under a quality management system to ensure it meets the stringent requirements of biopharmaceutical applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing specifications for activity, purity, and absence of specific contaminants. We can supply grades suitable for research use (RUO) and current Good Manufacturing Practice (cGMP) compliant material for therapeutic production.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C. This product is hygroscopic (moisture-sensitive). For long-term storage, consider desiccant or inert atmosphere to maintain optimal activity.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (Activity)	Conforms
Specific Activity	≥ 100 units/mg protein
Protein Content (Biuret)	≥ 80%
Solubility	Clear, colorless solution in specified buffer
Loss on Drying	≤ 5.0 %
Protease Activity (vs. Casein)	≤ 0.1 %

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.