Description

Pyrophosphatase is a crucial hydrolytic enzyme that catalyzes the cleavage of inorganic pyrophosphate (PPi) into two orthophosphate (Pi) molecules. This reaction is fundamental to driving energetically unfavorable biosynthetic reactions forward, making it an essential biocatalyst in numerous biochemical pathways. It is a vital reagent for research, diagnostic, and industrial applications in life sciences, molecular biology, and biocatalysis.

Application

• Molecular Biology & Biochemistry Research: Used in enzymatic assays, nucleotide sequencing, and studies of energy metabolism and signal transduction pathways.
• Diagnostic Kits & Reagents: A key component in clinical diagnostic assays, particularly for detecting nucleic acids (e.g., PCR, pyrosequencing) and various metabolites.
• Biocatalysis & Industrial Enzymology: Employed in synthetic biology and industrial processes to drive equilibrium-controlled reactions, such as polysaccharide and cofactor synthesis.
• Pharmaceutical Development: Utilized in drug discovery and development for target validation, high-throughput screening, and studying enzyme inhibition.
• Food & Beverage Analysis: Applied in enzymatic methods for food quality control and the detection of specific compounds like nucleotides.

Basic Information

Product Name	Pyrophosphatase
CAS No.	9024-82-2
Molecular Formula	N/A (Enzyme)
Molecular Weight	Varies by source and subunit composition
Synonyms	Inorganic Pyrophosphatase; Diphosphatase; PPase; Pyrophosphate Phospho-Hydrolase; EC 3.6.1.1; Tth Pyrophosphatase; Thermophilic Pyrophosphatase; Yeast Pyrophosphatase
EINECS	Contact for details

Quality Control

Our Pyrophosphatase is produced and tested under stringent quality management systems. Each lot undergoes comprehensive analysis to ensure high specific activity, purity, and consistency. A detailed Certificate of Analysis (COA) is provided, documenting parameters such as activity (U/mg), protein concentration, and absence of contaminating nucleases. We support compliance with relevant industry standards for research and in-vitro diagnostic use.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below in a non-frost-free freezer. The product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature before opening to minimize moisture condensation. For short-term use, aliquoting is recommended to avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	Lyophilized powder or solution
Identification (Activity)	Conforms
Specific Activity	> 1000 U/mg protein
Purity (SDS-PAGE)	> 95%
Contaminating Activities	Free of detectable RNase & DNase
Protein Concentration	Provided on COA
Buffer Composition	Provided on COA
Storage Buffer	Tris-HCl, Glycerol, DTT (as applicable)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.