Description

Aconitase CAS NO 9024-25-3 is a critical iron-sulfur enzyme that catalyzes the stereospecific isomerization of citrate to isocitrate in the tricarboxylic acid (TCA) cycle. This enzymatic activity is fundamental to cellular energy metabolism and bioenergetic research. It is essential for biochemical research, diagnostic kit manufacturing, and metabolic pathway analysis in the pharmaceutical and biotechnology industries.

Application

• Biochemical Research & Assay Development: Used as a key reagent in enzymatic studies of the Krebs cycle, mitochondrial function, and cellular respiration.
• Diagnostic Kit Manufacturing: Serves as a critical component in clinical diagnostic kits designed to measure citrate, isocitrate, or aconitase activity for metabolic disorder screening.
• Pharmaceutical R&D: Employed in drug discovery programs targeting metabolic diseases, cancer metabolism, and mitochondrial dysfunction.
• Enzyme Activity Studies: Utilized to investigate enzyme kinetics, inhibition, and the role of iron-sulfur clusters in catalytic mechanisms.
• Metabolic Pathway Analysis: Applied in vitro to reconstruct and study specific segments of the TCA cycle for metabolic engineering and systems biology.
• Quality Control Reference Standard: Acts as a reference standard in the quality control of related biochemical products and assay systems.

Basic Information

Product Name	Aconitase
CAS No.	9024-25-3
Molecular Formula	N/A (Enzyme)
Molecular Weight	~90-100 kDa (Monomer, species-dependent)
Synonyms	Aconitate Hydratase; Citrate(isocitrate) Hydro-lyase; EC 4.2.1.3; Aconitase 1; Iron-Responsive Element-Binding Protein 1; IRP1; Cytosolic Aconitase; Citrate Dehydratase; Mitochondrial Aconitase; ACO1; ACO2
EINECS	Contact for details

Quality Control

Our Aconitase is produced and tested under stringent quality management systems. Each lot undergoes rigorous analysis to ensure high specific activity, purity, and functional consistency. Certificates of Analysis (COA) detailing specific activity, purity (by SDS-PAGE), and stability are provided with every shipment to guarantee it meets the exacting requirements of research and diagnostic applications.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below in aliquots to avoid repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use. Handle under appropriate laboratory conditions.

Specification

Item	Specification
Appearance	Lyophilized powder or solution
Specific Activity	≥ 10 units/mg protein
Purity (SDS-PAGE)	≥ 90%
Protein Content (Biuret)	Contact for details
Identification (Activity)	Conforms
pH (in solution)	7.0 - 7.5
Stabilizers/Additives	May contain buffer salts (e.g., Tris, phosphate)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.