Description

Apotryptophanase CAS NO 9024-00-4 is a highly purified enzyme preparation, specifically a tryptophanase, used in biochemical research and industrial biocatalysis. This product is essential for researchers and manufacturers requiring precise enzymatic activity for the synthesis, degradation, or analysis of tryptophan and related indole compounds. It is a critical reagent for pharmaceutical development, diagnostic assay manufacturing, and advanced life science research.

Application

• Biochemical Research: Used as a model enzyme in studies of pyridoxal phosphate-dependent catalysis, enzyme kinetics, and protein structure-function relationships.
• Diagnostic Reagent Manufacturing: A key component in enzymatic assay kits for the clinical and research-based quantification of L-tryptophan or indole.
• Pharmaceutical Intermediates: Employed in the biocatalytic synthesis of chiral intermediates and active pharmaceutical ingredients (APIs) where tryptophan metabolism is relevant.
• Microbiology & Metabolic Studies: Utilized in research to study bacterial metabolism, specifically the tryptophanase operon and indole signaling pathways in microorganisms.
• Food & Feed Analysis: Applied in enzymatic methods for determining tryptophan content in nutritional products, supplements, and animal feed.
• Enzyme Engineering: Serves as a starting point for protein engineering projects aimed at developing tryptophanase variants with altered substrate specificity or enhanced stability.

Basic Information

Product Name	Apotryptophanase
CAS No.	9024-00-4
Molecular Formula	N/A (Enzyme)
Molecular Weight	~220 kDa (Tetramer)
Synonyms	Tryptophanase; L-Tryptophan Indole-Lyase (Deaminating); Tryptophan Indole-Lyase; EC 4.1.99.1; TNAase; L-Tryptophanase; Tryptophan Deaminase; Apotryptophanase (Apoenzyme Form)
EINECS	232-795-1

Quality Control

Our Apotryptophanase is produced under stringent quality management systems. Each batch is tested to ensure high specific activity, purity, and consistency. Quality parameters include protein content (Lowry/Bradford), specific activity (U/mg), and absence of contaminating protease activities. A comprehensive Certificate of Analysis (COA) detailing assay results, physical appearance, and stability data is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below. This product is hygroscopic (moisture-sensitive); allow the vial to equilibrate to room temperature before opening to prevent condensation. Aliquot reconstituted enzyme to avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	Lyophilized powder or suspension
Identification (Activity)	Catalyzes the degradation of L-Tryptophan
Protein Content (Bradford)	> 90%
Specific Activity	> 50 units/mg protein
pH Optimum	7.0 - 8.5
Temperature Optimum	37°C
Pyridoxal Phosphate (PLP) Dependency	Requires exogenous PLP for full activity
Stability	> 90% activity retained after 12 months at -20°C

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.