Description

D-Ribose-5-Phosphate Ketol-Isomerase Type I is a crucial enzyme that catalyzes the isomerization of D-ribose-5-phosphate to D-xylulose-5-phosphate, a key step in the pentose phosphate pathway. This enzymatic activity is fundamental for research in carbohydrate metabolism, nucleotide synthesis, and cellular energy production. It is essential for biochemical research laboratories, pharmaceutical development teams, and diagnostic manufacturers focusing on metabolic pathway analysis and enzyme kinetics studies.

Application

• Biochemical Research: Used as a critical reagent for in vitro studies of the pentose phosphate pathway, carbohydrate metabolism, and enzymatic mechanisms.
• Pharmaceutical Development: Employed in target validation and screening assays for metabolic disorders, cancer research, and diseases linked to pentose phosphate pathway dysregulation.
• Diagnostic Kit Manufacturing: Serves as a key component in enzymatic assay kits designed to measure metabolic intermediates or diagnose specific enzyme deficiencies.
• Enzyme Kinetics Studies: Utilized to determine kinetic parameters (Km, Vmax) and study inhibitor effects, providing data for drug discovery and basic science.
• Metabolic Engineering: Applied in synthetic biology and metabolic engineering research to understand and manipulate sugar metabolism in microorganisms.
• Educational & Teaching Tools: Supplied to academic institutions for advanced biochemistry laboratory courses and practical demonstrations of isomerase activity.

Basic Information

Product Name	D-Ribose-5-Phosphate Ketol-Isomerase Type I
CAS No.	9023-83-0
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Ribose-5-phosphate isomerase; RpiA; Phosphoriboisomerase; D-ribose-5-phosphate aldose-ketose-isomerase; 5-phosphoribose isomerase; EC 5.3.1.6; RPI; Ribose phosphate isomerase
EINECS	Contact for details

Quality Control

Our D-Ribose-5-Phosphate Ketol-Isomerase Type I is produced and tested under stringent quality management systems. Each lot undergoes rigorous analytical testing to ensure high specific activity, purity, and stability. Certificates of Analysis (COA) detailing assay results, protein content, and activity units are provided with every shipment to guarantee batch-to-batch consistency for your research and development needs.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below. This product is hygroscopic (moisture-sensitive). Avoid repeated freeze-thaw cycles. Keep container tightly sealed in a dry environment.

Specification

Item	Specification
Appearance	Lyophilized powder or solution
Specific Activity	≥ [Value] units/mg protein
Protein Content (Biuret)	Contact for details
Purity (SDS-PAGE)	≥ 90%
pH (in solution)	7.0 - 7.5
Stabilizers / Buffers	May contain glycerol, buffer salts
Biological Activity	Functional in standard assay conditions

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.