Description

Adenylosuccinate Synthase is a critical enzyme in the purine nucleotide biosynthetic pathway, specifically catalyzing the formation of adenylosuccinate from IMP and aspartate. This makes it an essential reagent for biochemical research, metabolic pathway analysis, and the development of diagnostic assays. It is primarily utilized by researchers and manufacturers in the fields of pharmaceutical development, enzymology, and molecular biology to study nucleotide metabolism and related disorders.

Application

• Biochemical Research: Essential for in vitro studies of the de novo purine biosynthesis pathway and enzyme kinetics.
• Diagnostic Kit Development: Used as a key component in enzymatic assays for diagnosing inborn errors of metabolism, such as adenylosuccinate lyase deficiency.
• Pharmaceutical Screening: Serves as a target enzyme for screening potential inhibitors in drug discovery programs for cancer, microbial infections, and autoimmune diseases.
• Enzyme Production & Purification: Acts as a standard or reference material in the production and quality control of related enzymatic products.
• Academic & Educational Use: Employed in university and institutional laboratories for teaching advanced concepts in biochemistry and enzymology.

Basic Information

Product Name	Adenylosuccinate Synthase
CAS No.	9023-57-8
Molecular Formula	N/A (Enzyme)
Molecular Weight	Varies by source and purification (typically ~50 kDa monomer)
Synonyms	IMP–aspartate ligase; Adenylosuccinate synthetase; AMPSase; AdSS; PurB (in some contexts); EC 6.3.4.4
EINECS	Contact for details

Quality Control

Our Adenylosuccinate Synthase is produced and tested under stringent conditions to ensure high activity, purity, and batch-to-batch consistency suitable for research and diagnostic applications. Each lot is characterized for specific activity and purity profile. Certificates of Analysis (COA) detailing activity units/mg protein, purity by SDS-PAGE, and absence of contaminating nucleases/proteases are available upon request.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below in aliquots to avoid repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use. Handle under appropriate laboratory conditions.

Specification

Item	Specification
Appearance	Lyophilized powder or solution in buffer
Specific Activity	≥ [Value] units/mg protein
Purity (SDS-PAGE)	≥ 90%
Protein Concentration	As per COA
Buffer Composition	Tris-HCl, Glycerol, DTT (or as specified)
Contaminants	Free of detectable DNase, RNase, and protease activity

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.