Description

β-Hydroxysteroid Dehydrogenase is a critical enzyme belonging to the oxidoreductase family, specifically catalyzing the reversible oxidation/reduction of hydroxyl and ketone groups on steroid molecules. This biochemical activity makes it an indispensable tool for research and development in steroid metabolism and hormone synthesis pathways. It is primarily utilized by pharmaceutical R&D laboratories, academic research institutions, and biotechnology companies focused on endocrinology, metabolic disease studies, and the development of novel therapeutic agents.

Application

• Biochemical Research & Assay Development: Used as a key reagent in enzymatic assays to study steroid hormone biosynthesis and metabolism in vitro.
• Pharmaceutical R&D: Employed in the investigation of metabolic pathways for drug discovery targeting hormonal disorders, cancers, and metabolic syndromes.
• Diagnostic Kit Manufacturing: Serves as a component in the development of diagnostic kits for measuring specific steroid hormone levels in clinical samples.
• Enzymology Studies: Utilized to understand enzyme kinetics, inhibition mechanisms, and structure-function relationships within the hydroxysteroid dehydrogenase family.
• Academic & Industrial Biotechnology: Applied in metabolic engineering projects aimed at the microbial or enzymatic production of valuable steroid intermediates.

Basic Information

Product Name	β-Hydroxysteroid Dehydrogenase
CAS No.	9015-81-0
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	3β-Hydroxysteroid Dehydrogenase; 3β-HSD; 3-β-HSD; δ5-3β-Hydroxysteroid Dehydrogenase; 3β-Hydroxy-δ5-steroid Dehydrogenase; EC 1.1.1.145; Steroid 3β-Dehydrogenase; 3β-Hydroxysteroid Oxidoreductase
EINECS	Contact for details

Quality Control

Our β-Hydroxysteroid Dehydrogenase is produced and tested under stringent quality management protocols to ensure high purity, specific activity, and batch-to-batch consistency suitable for research and industrial applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing parameters such as specific activity, protein content, purity, and stability. We adhere to relevant quality standards, and specifications can be tailored to meet the requirements of cGMP, ISO, or other regulatory frameworks as needed.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below for long-term stability. This material is hygroscopic (moisture-sensitive); ensure containers are sealed tightly after use to prevent moisture absorption. For short-term use, aliquoting is recommended to avoid repeated freeze-thaw cycles, which can degrade enzymatic activity.

Specification

Item	Specification
Appearance	Lyophilized powder or solution
Specific Activity	≥ [Value] units/mg protein
Protein Content (Biuret)	Contact for details
Purity (SDS-PAGE)	≥ 90%
pH Optimum	~7.0 - 9.0
Temperature Optimum	~37°C
Stabilizers / Buffers	May contain glycerol, buffer salts

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.