Description

Enterokinase is a highly specific serine protease that catalyzes the activation of trypsinogen to trypsin, serving as a critical enzyme in protein digestion and recombinant protein processing. Its precise cleavage after the recognition sequence (Asp-Asp-Asp-Asp-Lys) makes it an indispensable tool for the removal of affinity tags in biopharmaceutical production. This product is essential for research institutions, diagnostic kit manufacturers, and biotechnology companies engaged in protein engineering, structural biology, and therapeutic protein development. We supply high-purity Enterokinase to support consistent and reliable results in demanding applications.

Application

• Recombinant Protein Cleavage: Specific removal of fusion tags (e.g., His-tag, GST, MBP) from expressed proteins without damaging the target protein sequence.
• Biopharmaceutical Manufacturing: Critical downstream processing step for the activation of therapeutic proteins and peptides.
• Proteomics & Biochemical Research: Used in enzymatic assays, zymogen activation studies, and protein digestion protocols.
• Diagnostic Reagent Production: Component in diagnostic kits requiring specific proteolytic activation for signal generation.
• Structural Biology: Preparation of protein samples for crystallography and NMR by generating native protein termini.
• Cell Culture & Biotechnology: Processing of growth factors and other bioactive proteins in cell-based applications.

Basic Information

Product Name	Enterokinase
CAS No.	9014-74-8
Molecular Formula	N/A (Protein)
Molecular Weight	~150 kDa (Light Chain ~46 kDa, Heavy Chain ~104 kDa)
Synonyms	Enteropeptidase; Kinase, Entero; Enterokinase (light chain); Duodenase; EC 3.4.21.9; Trypsinogen activator; EK protease
EINECS	Contact for details

Quality Control

Our Enterokinase is manufactured and tested under stringent quality systems to ensure high purity, specific activity, and lot-to-lot consistency suitable for research and industrial use. Each batch is accompanied by a comprehensive Certificate of Analysis (COA) detailing key parameters such as specific activity, purity level (by SDS-PAGE), and absence of contaminating proteases. We adhere to relevant quality guidelines to support our customers' regulatory and compliance needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below for long-term stability. For short-term use, aliquots may be stored at 2-8°C. The product is hygroscopic (moisture-sensitive); allow the vial to equilibrate to room temperature before opening to prevent condensation. Avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	Lyophilized powder or solution
Identification (Activity)	Cleaves specific fusion tag sequences
Specific Activity	> X units/mg protein (Contact for details)
Purity (SDS-PAGE)	> 90%
Protease Contamination	Undetectable levels of trypsin, chymotrypsin
Protein Concentration	Determined by UV absorbance
Buffer Composition	Tris-HCl, Calcium Chloride, Glycerol
pH	7.4 - 8.0

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.