Description

Tryptophan Synthase is a critical pyridoxal phosphate-dependent enzyme complex that catalyzes the final two steps in the biosynthesis of the essential amino acid L-tryptophan. This biocatalyst is indispensable for research and industrial processes focused on amino acid metabolism, enzyme kinetics, and the production of high-value biochemicals. It is primarily utilized by biotechnology firms, academic research institutions, and pharmaceutical developers engaged in metabolic engineering, biochemical assay development, and the synthesis of tryptophan-derived compounds.

Application

• Biochemical Research & Assay Development: Essential for studying the tryptophan biosynthetic pathway, enzyme mechanism, and allosteric regulation.
• Pharmaceutical Intermediate Synthesis: Used in enzymatic cascades for the production of L-tryptophan and its derivatives, which serve as precursors for various active pharmaceutical ingredients (APIs).
• Metabolic Engineering: A key enzyme for incorporation into microbial hosts (e.g., E. coli, yeast) to engineer optimized pathways for tryptophan overproduction.
• Diagnostic Reagent Manufacturing: Employed in the formulation of diagnostic kits and reagents for clinical and research laboratories to measure related metabolites or enzyme activity.
• Enzyme Structure-Function Studies: Serves as a model system for X-ray crystallography and mutagenesis studies due to its well-characterized α2β2 tetrameric structure.
• Nutritional Supplement Production: Applied in enzymatic synthesis processes for the manufacture of high-purity L-tryptophan used in animal feed and human nutritional supplements.

Basic Information

Product Name	Tryptophan Synthase
CAS No.	9014-52-2
Molecular Formula	N/A (Enzyme)
Molecular Weight	~143 kDa (α2β2 complex)
Synonyms	Tryptophan synthase; L-Serine hydro-lyase (adding indole); Tryptophan synthetase; TS; TRPS; EC 4.2.1.20; Indoleglycerol phosphate lyase; α subunit (TrpA); β subunit (TrpB)
EINECS	Contact for details

Quality Control

Our Tryptophan Synthase is produced and tested under stringent conditions to ensure batch-to-batch consistency and high enzymatic activity. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing specific activity, purity, and stability parameters. We adhere to relevant quality management standards to support research and cGMP manufacturing needs.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below in aliquots to avoid repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use. Store away from strong acids, bases, and oxidizing agents.

Specification

Item	Specification
Appearance	Lyophilized powder or solution
Identification (Activity)	Conforms
Specific Activity	≥ [Value] units/mg protein
Protein Content (Biuret)	≥ [Value] %
Purity (SDS-PAGE)	≥ 90%
pH (in solution)	7.0 - 7.5
Endotoxin Level	< 1.0 EU/mg (Research grade)
Storage Buffer	Tris-HCl or Phosphate buffer

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.