Description

Polynucleotide Phosphorylase From Bacillus (PNPase) is a highly specific enzyme that catalyzes the reversible phosphorolysis of RNA, a critical reaction in nucleotide metabolism and RNA processing. This product is essential for researchers and manufacturers requiring a reliable, high-activity enzyme for synthesizing defined-sequence polynucleotides or studying RNA degradation pathways. It is primarily utilized in molecular biology research, diagnostic kit development, and the production of nucleotide-based biochemicals.

Application

• Molecular Biology Research: Used in the study of RNA metabolism, degradation, and turnover mechanisms.
• In Vitro RNA Synthesis: Catalyzes the template-independent polymerization of ribonucleoside diphosphates (NDPs) to generate homo- or heteropolymers.
• Diagnostic Reagent Manufacturing: A key component in enzymatic assays and kits for nucleotide analysis and RNA-related diagnostics.
• Biochemical Synthesis: Employed in the enzymatic production of specific oligonucleotides and nucleotide analogs.
• Enzymology Studies: Serves as a model enzyme for investigating phosphorylase kinetics and mechanism.
• Educational & Laboratory Use: Supplied to academic and industrial laboratories for standard enzymatic procedures.

Basic Information

Product Name	Polynucleotide Phosphorylase From Bacillus
CAS No.	9014-12-4
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	PNPase; Polynucleotide Phosphorylase (Bacillus); Polyribonucleotide Nucleotidyltransferase; EC 2.7.7.8; RNA Phosphorylase; Nucleoside Diphosphate:Polyribonucleotide Nucleotidyltransferase; PNP; Bacillus PNPase
EINECS	Contact for details

Quality Control

Our Polynucleotide Phosphorylase is produced and tested under stringent quality management systems to ensure batch-to-batch consistency and high enzymatic activity. Each lot is characterized for specific activity, purity, and absence of contaminating nucleases. A comprehensive Certificate of Analysis (COA) detailing assay results, purity profiles, and stability data is provided with every shipment to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below. Avoid repeated freeze-thaw cycles. The product should be kept under the recommended conditions to maintain optimal enzymatic activity.

Specification

Item	Specification
Appearance	Lyophilized powder or solution
Identification (Enzyme Activity)	Conforms
Specific Activity	> XX units/mg protein
Protein Content (Biuret)	> XX %
Purity (SDS-PAGE)	> XX %
Contaminating Nuclease Activity	Not detected
pH (in solution)	7.0 - 8.0
Solubility	Completely soluble in appropriate buffer

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.