Description

Enolase is a crucial glycolytic enzyme that catalyzes the dehydration of 2-phosphoglycerate to phosphoenolpyruvate, a key step in energy metabolism. This product is essential for research and development in biochemistry, molecular biology, and diagnostic applications. It is primarily utilized by biotechnology firms, academic research institutions, and manufacturers of diagnostic kits and reagents.

Application

• Biochemical Research: Used as a critical reagent in enzymatic studies, metabolic pathway analysis, and protein interaction research.
• Diagnostic Kit Manufacturing: Serves as a key component in clinical diagnostic assays, particularly for measuring metabolites like pyruvate or related enzymatic activities.
• Enzyme Standardization: Employed as a reference standard for calibrating equipment and validating assay methods in laboratory settings.
• Pharmaceutical Development: Investigated in drug discovery for targeting metabolic pathways in areas such as oncology and infectious diseases.
• Food & Beverage Analysis: Used in enzymatic methods for the quantitative determination of sugars and other carbohydrates in quality control labs.
• Educational Purposes: Supplied to universities and teaching laboratories for practical demonstrations of enzyme kinetics and glycolysis.

Basic Information

Product Name	Enolase
CAS No.	9014-08-8
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	2-Phospho-D-glycerate hydro-lyase; Phosphopyruvate hydratase; 2-Phosphoglycerate dehydratase; EC 4.2.1.11; Muscle enolase; Neural enolase; Non-neuronal enolase; Enolase 1; Enolase 2; Enolase 3
EINECS	Contact for details

Quality Control

Our Enolase is produced and tested under stringent quality management systems. Each batch undergoes rigorous analytical testing to ensure high purity, specific activity, and consistency. Certificates of Analysis (COA) detailing parameters such as activity units/mg protein, purity by SDS-PAGE, and absence of contaminating protease activities are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C. The product is hygroscopic (moisture-sensitive). For long-term storage, consider keeping the container in a desiccator. Avoid repeated freeze-thaw cycles of prepared solutions.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (IR)	Conforms
Specific Activity	≥ 50 units/mg protein
Protein Content (Biuret)	≥ 90%
Purity (SDS-PAGE)	≥ 95%
pH (1% solution)	6.0 - 7.5
Loss on Drying	≤ 5.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.