Description

Ec 1.14.14.3 is a cytochrome P450 monooxygenase enzyme, a critical biocatalyst in oxidative metabolic pathways. This enzyme matters for its role in the selective hydroxylation of a wide range of organic substrates, enabling the synthesis of complex molecules and the study of drug metabolism. Industries that require precise, enzyme-driven oxidation processes, such as pharmaceutical R&D, biotechnology, and fine chemical synthesis, need this high-purity biological reagent.

Application

• Pharmaceutical Research & Development: Used as a model enzyme for studying Phase I drug metabolism, toxicity screening, and metabolite identification.
• Biocatalysis & Fine Chemical Synthesis: Employed in the enzymatic synthesis of chiral intermediates, steroids, and other high-value compounds via selective C-H activation.
• Biotechnology & Enzyme Engineering: Serves as a target protein for directed evolution studies aimed at improving catalytic activity, stability, or substrate specificity.
• Academic & Institutional Research: Utilized in biochemical studies to investigate electron transfer mechanisms, structure-function relationships, and heme-protein interactions.
• Diagnostic Kit Development: Can be incorporated into in vitro assay systems for detecting specific enzymatic activities or metabolic disorders.

Basic Information

Product Name	Ec 1.14.14.3
CAS No.	9014-00-0
Molecular Formula	Protein
Molecular Weight	Varies by isoform and source (typically 50-60 kDa)
Synonyms	Cytochrome P450 Monooxygenase; Unspecific Monooxygenase; Aryl-4-monooxygenase; CYP Enzyme; Heme-thiolate Protein P450; Microsomal Cytochrome P450; Xenobiotic Monooxygenase; Drug-Metabolizing Enzyme
EINECS	Contact for details

Quality Control

Our Ec 1.14.14.3 is produced and tested under stringent conditions to ensure high biological activity and purity. Each batch is characterized for specific activity, protein content, and spectral properties (P450 CO-difference spectrum). A comprehensive Certificate of Analysis (COA) detailing purity (by SDS-PAGE), activity units, and storage buffer composition is provided to guarantee reproducible performance in your research and development applications.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store frozen at -20°C to -80°C in small aliquots to avoid repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive); ensure the container is sealed properly after each use. Handle under appropriate laboratory conditions.

Specification

Item	Specification
Appearance	Clear to slightly opalescent solution
Identification (Spectral)	Characteristic P450 CO-difference spectrum present
Protein Concentration	≥ 1.0 mg/mL (by Bradford or similar)
Specific Activity	≥ 5.0 nmol product formed/min/mg protein (substrate-dependent)
Purity (SDS-PAGE)	≥ 90% (single band corresponding to ~55 kDa)
Storage Buffer	50 mM Potassium Phosphate, pH 7.4, 20% Glycerol, 1 mM EDTA

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.