Description

Malate Synthase From Bacillus Stearothermophilus is a highly thermostable enzyme that catalyzes the condensation of glyoxylate and acetyl-CoA to form malate, a key reaction in the glyoxylate cycle. This robust biocatalyst is essential for research and industrial processes requiring high-temperature stability and consistent enzymatic activity. It is primarily utilized by biochemists, molecular biologists, and process engineers in academic research, metabolic pathway analysis, and the development of biocatalytic synthesis platforms.

Application

• Biochemical Research & Metabolic Studies: Essential for in vitro studies of the glyoxylate cycle, gluconeogenesis, and microbial metabolism.
• Enzyme Kinetics & Characterization: Used as a model thermostable enzyme for studying protein stability, catalytic mechanisms, and structure-function relationships.
• Diagnostic Kit Manufacturing: Serves as a key component in enzymatic assay kits for clinical and research diagnostics targeting specific metabolic pathways.
• Biocatalysis & Synthetic Biology: Employed in engineered metabolic pathways for the biosynthesis of organic acids, amino acids, and other valuable compounds.
• Industrial Biotechnology: Potential application in bioremediation and biofuel production processes that benefit from thermostable enzymatic activity.
• Educational Purposes: Used in advanced university laboratory courses for teaching enzymology and metabolic biochemistry.

Basic Information

Product Name	Malate Synthase From Bacillus Stearothermophilus
CAS No.	9013-48-3
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Malate Synthase (B. stearothermophilus); L-Malate Synthase; Glyoxylate Condensing Enzyme; (S)-Malate Glyoxylate-Lyase (CoA-acetylating); EC 2.3.3.9; MS; Thermostable Malate Synthase
EINECS	Contact for details

Quality Control

Our Malate Synthase is produced and tested under stringent quality management protocols to ensure batch-to-batch consistency and high specific activity. Each lot is characterized for purity, activity, and stability. Certificates of Analysis (COA) documenting detailed specifications are provided with every shipment to support your quality assurance and regulatory compliance needs.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below. This product is hygroscopic (moisture-sensitive); allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	Lyophilized powder or solution
Identification (Activity)	Conforms
Specific Activity	≥ [Value] units/mg protein
Protein Content (Biuret)	≥ [Value] %
Purity (SDS-PAGE)	≥ [Value] %
pH (in solution)	6.0 - 8.0
Endotoxin (LAL)	< [Value] EU/mg

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.