Description

Adenylate Kinase is a crucial phosphotransferase enzyme that catalyzes the interconversion of adenine nucleotides, playing a vital role in cellular energy homeostasis. This enzyme is essential for maintaining the energetic balance of ATP, ADP, and AMP within cells, making it a fundamental tool for biochemical research and diagnostic applications. It is primarily utilized by researchers in biochemistry, molecular biology, and pharmaceutical development, as well as by manufacturers of diagnostic kits and reagents for clinical testing.

Application

• Biochemical Research & Assay Development: Used as a key reagent in enzymatic assays to study energy metabolism, kinase activity, and nucleotide-dependent processes.
• Diagnostic Kit Manufacturing: A critical component in commercial diagnostic kits for measuring ATP, ADP, and AMP levels, particularly in clinical chemistry for cardiac and muscular function tests.
• Pharmaceutical Screening: Employed in high-throughput screening (HTS) platforms to identify potential modulators of nucleotide metabolism for drug discovery.
• Enzyme Kinetics Studies: Serves as a model enzyme for studying phosphotransferase mechanisms, enzyme catalysis, and inhibitor interactions.
• Metabolic Pathway Analysis: Used in vitro to reconstruct and analyze segments of cellular metabolic pathways related to energy transfer.
• Reference Standard: Acts as a purity and activity standard for quality control in the production of other enzymatic reagents.

Basic Information

Product Name	Adenylate Kinase
CAS No.	9013-02-9
Molecular Formula	N/A (Protein)
Molecular Weight	~21-25 kDa (monomer, varies by source)
Synonyms	AK; ATP-AMP Transphosphorylase; Myokinase; Adenylate Monophosphate Kinase; AMP Kinase; ADP:ATP Phosphotransferase; EC 2.7.4.3; 5'-Adenylic Acid Kinase
EINECS	Contact for details

Quality Control

Our Adenylate Kinase is produced and tested under stringent quality management systems. Each batch undergoes rigorous analysis to ensure high specific activity, purity, and stability. Certificates of Analysis (COA) detailing parameters such as activity (U/mg), protein content, and purity are available upon request. Production can adhere to relevant guidelines for research and diagnostic use.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below. The product is hygroscopic (moisture-sensitive); allow the vial to equilibrate to room temperature before opening to prevent condensation. Avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder or solution
Identification (Activity)	Conforms (Enzymatic Assay)
Specific Activity	> 500 units/mg protein
Purity (SDS-PAGE)	> 95%
Protein Content (Biuret)	Contact for details
pH (in solution)	7.0 - 7.5
Endotoxin (LAL)	< 1.0 EU/μg (if specified)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.