Description

Dna Polymerase CAS NO 9012-90-2 is a critical enzyme that catalyzes the synthesis of DNA molecules from deoxyribonucleotides, serving as the fundamental engine for DNA replication and amplification. Its precision and processivity are paramount for ensuring high-fidelity genetic copying, making it an indispensable component in modern molecular biology. This enzyme is essential for researchers, diagnostic developers, and biotech manufacturers engaged in polymerase chain reaction (PCR), DNA sequencing, cloning, and various genetic analysis techniques.

Application

• Polymerase Chain Reaction (PCR): The primary application for amplifying specific DNA sequences in research, diagnostics, and forensic analysis.
• DNA Sequencing: Used in Sanger (chain-termination) and next-generation sequencing (NGS) platforms for determining nucleotide order.
• Molecular Cloning: Essential for synthesizing DNA inserts and performing site-directed mutagenesis.
• cDNA Synthesis: Employed in reverse transcription PCR (RT-PCR) for generating complementary DNA from RNA templates.
• Genotyping and Mutagenesis: Critical for identifying genetic variations and creating specific mutations for functional studies.
• Diagnostic Test Kits: A key reagent in commercial kits for detecting pathogens, genetic disorders, and biomarkers.
• Biotechnology & Pharmaceutical Development: Used in gene synthesis, vaccine development, and therapeutic protein production.

Basic Information

Product Name	Dna Polymerase
CAS No.	9012-90-2
Molecular Formula	N/A (Protein/Enzyme)
Molecular Weight	Varies by source and type (e.g., ~94 kDa for Taq polymerase)
Synonyms	DNA Polymerase; DNA Nucleotidyltransferase; DNA-Directed DNA Polymerase; Taq Polymerase; Pfu Polymerase; Klenow Fragment; T4 DNA Polymerase; DNA Replicase; EC 2.7.7.7
EINECS	Contact for details

Quality Control

Our Dna Polymerase is produced and tested under stringent quality management systems to ensure batch-to-batch consistency, high activity, and purity. Each lot is characterized for critical parameters including specific activity, fidelity, processivity, and the absence of contaminating nucleases. Certificates of Analysis (COA) detailing purity, activity units, and functional performance data are provided with every shipment. We support compliance with relevant ISO and GMP guidelines for research and diagnostic use.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C in a non-frost-free freezer. Avoid repeated freeze-thaw cycles. The enzyme is typically supplied in a buffered glycerol solution to maintain stability at freezing temperatures. Always handle on ice or in a cold block when thawed for use.

Specification

Item	Specification
Appearance	Clear, colorless solution
Identification (Activity)	Catalyzes DNA-directed DNA synthesis
Purity (SDS-PAGE)	≥ 95%
Specific Activity	Contact for details (Units/mg)
Exonuclease Activity	Absent (or specified for proofreading types)
Endonuclease Activity	Not detected
RNase Activity	Not detected
Functional Test (PCR)	Amplifies target band of correct size
Buffer Composition	Tris-HCl, Glycerol, DTT, EDTA, Stabilizers

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.