Description

Acetyl-Coa Synthetase CAS NO 9012-31-1 is a critical enzyme that catalyzes the ATP-dependent formation of acetyl-CoA from acetate, CoA, and ATP, playing a fundamental role in cellular metabolism. This biocatalyst is essential for research and industrial processes involving lipid biosynthesis, energy production, and the citric acid cycle. It is a vital reagent for biochemists, molecular biologists, and manufacturers in the pharmaceutical, biotechnology, and diagnostic kit development sectors.

Application

• Biochemical Research & Assay Development: Used as a key component in enzymatic assays to study metabolic pathways, enzyme kinetics, and cellular energy regulation.
• Pharmaceutical Manufacturing: Employed in the synthesis of complex molecules and as a tool in metabolic engineering for drug discovery and production.
• Diagnostic Kit Production: Integrated into in-vitro diagnostic (IVD) kits for the detection and quantification of metabolites related to metabolic disorders.
• Academic & Industrial Enzymology: Serves as a standard enzyme for teaching and research in university and corporate R&D laboratories.
• Biofuel & Bioprocess Development: Utilized in metabolic engineering strategies to optimize microbial strains for the production of biofuels and biochemicals from acetate.
• Food & Nutrition Research: Applied in studies investigating fat metabolism, energy balance, and the biochemical basis of nutrition.

Basic Information

Product Name	Acetyl-Coa Synthetase
CAS No.	9012-31-1
Molecular Formula	N/A (Enzyme)
Molecular Weight	Varies by source and preparation
Synonyms	Acetate-CoA ligase; Acetyl-CoA synthetase; ACS; Acetyl coenzyme A synthetase; Acetate thiokinase; Acetyl-activating enzyme; EC 6.2.1.1; Acetyl-CoA ligase
EINECS	Contact for details

Quality Control

Our Acetyl-Coa Synthetase is produced and tested under stringent quality management systems. Each lot undergoes rigorous analytical testing to ensure high specific activity, purity, and stability. Certificates of Analysis (COA) detailing parameters such as activity (U/mg), protein content, and purity are provided to guarantee batch-to-batch consistency for your critical applications.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below. This product is hygroscopic (moisture-sensitive); allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	Lyophilized powder or solution
Specific Activity	≥ [Value] units/mg protein
Protein Content (Biuret)	Contact for details
Purity (SDS-PAGE)	≥ 90%
Stabilizers/Additives	May contain buffer salts (e.g., phosphate, Tris) and stabilizers (e.g., glycerol)
Solubility	Soluble in appropriate aqueous buffers
Storage Buffer	Contact for details

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.