Description

Dihydrofolate Reductase F. Bovine Liver, ~8 U/Mg, 500 Ul is a highly purified enzyme preparation isolated from bovine liver tissue. This product is essential for biochemical research and diagnostic applications requiring the specific catalysis of dihydrofolate reduction to tetrahydrofolate. It is a critical reagent for scientists and manufacturers in the pharmaceutical, life science, and in-vitro diagnostic (IVD) industries. The enzyme's activity and purity make it suitable for demanding applications in enzyme kinetics studies, metabolic pathway analysis, and high-throughput screening assays.

Application

• Biochemical Research & Enzyme Kinetics: Used as a standard enzyme for studying inhibition mechanisms, reaction rates, and cofactor dependencies in folate metabolism.
• Pharmaceutical Drug Discovery: Serves as a key target enzyme in high-throughput screening (HTS) assays for the development of novel antifolate drugs, including antimicrobial and anticancer agents.
• In-Vitro Diagnostic (IVD) Kits: Incorporated as a critical component in diagnostic test kits designed to measure folate levels or assess metabolic disorders related to folate cycle enzymes.
• Metabolic Pathway Analysis: Utilized in reconstituted enzymatic systems to investigate the folate cycle and its interplay with nucleotide synthesis and amino acid metabolism.
• Educational & Reference Standards: Employed in academic and industrial laboratory settings for teaching advanced enzymology and as a quality control standard.
• Enzyme-Linked Assay Development: Acts as a coupling enzyme in spectrophotometric or fluorometric assays to monitor the activity of other enzymes within related biochemical pathways.

Basic Information

Item	Details
Product Name	Dihydrofolate Reductase F. Bovine Liver, ~8 U/Mg, 500 Ul
CAS No.	9002-03-3
Molecular Formula	Protein (Enzyme)
Molecular Weight	~20-25 kDa (monomer, approximate)
Synonyms	DHFR (Bovine Liver); Dihydrofolate Reductase (NADPH) from Bovine Liver; Tetrahydrofolate Dehydrogenase; 5,6,7,8-Tetrahydrofolate:NADP+ Oxidoreductase; Folate Reductase; EC 1.5.1.3; Dihydrofolic Acid Reductase
EINECS	Contact for details

Quality Control

Our Dihydrofolate Reductase is manufactured under controlled conditions to ensure high specific activity and purity. Each lot undergoes rigorous analytical testing, including activity assay, protein content determination, and purity assessment via electrophoresis. A comprehensive Certificate of Analysis (COA) detailing specific activity, protein concentration, and buffer composition is provided with every shipment to guarantee batch-to-batch consistency for your critical research and production needs.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store frozen at -20°C or below. Avoid repeated freeze-thaw cycles. The product is typically supplied in a buffered glycerol solution to maintain stability during storage.

Specification

Item	Specification
Appearance	Clear to slightly opalescent solution
Specific Activity	~8 U/mg protein (minimum)
Protein Concentration	Contact for details
Purity (SDS-PAGE)	>90%
Activity (per vial)	500 U (nominal)
Buffer	pH 7-8, containing stabilizer (e.g., glycerol)
Identification (Activity)	Conforms

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.