Description

Ec 5.4.2.2 CAS NO 9001-81-4 is a high-purity enzyme preparation, specifically phosphoglucomutase, which is critical for carbohydrate metabolism pathways. This product is essential for research and industrial applications requiring precise biocatalytic activity for the interconversion of glucose-1-phosphate and glucose-6-phosphate. It is primarily utilized by biotechnology firms, diagnostic kit manufacturers, and academic research institutions focused on enzymology, metabolic engineering, and biochemical assay development.

Application

• Biochemical Research & Assay Development: Used as a key reagent in enzymatic assays to study glycogen metabolism and glycolytic pathways.
• Diagnostic Manufacturing: Incorporated into clinical diagnostic kits for the measurement of glucose and related metabolites.
• Enzyme Engineering: Serves as a model enzyme or starting material for protein engineering studies aimed at improving thermal stability or catalytic efficiency.
• Biocatalysis: Employed in specialized synthetic chemistry applications for the stereoselective synthesis of sugar phosphates.
• Food & Beverage Analysis: Used in enzymatic methods for the accurate quantification of starch and glycogen content in food products.
• Pharmaceutical Intermediates: Potential use in the enzymatic synthesis of carbohydrate-based pharmaceutical intermediates.

Basic Information

Product Name	Ec 5.4.2.2 CAS NO 9001-81-4
CAS No.	9001-81-4
Molecular Formula	Protein (Enzyme)
Molecular Weight	Varies by source and preparation (approx. 60-65 kDa monomer)
Synonyms	Phosphoglucomutase; PGM; α-D-Glucose 1,6-phosphomutase; Glucose phosphomutase; D-Glucose 1,6-phosphomutase; PGM 1; EC 5.4.2.2; Phosphohexomutase
EINECS	232-650-1

Quality Control

Our phosphoglucomutase is produced and tested under strict quality management systems. Each lot undergoes comprehensive analysis to ensure high specific activity, purity, and functional consistency. Certificates of Analysis (COA) are provided, detailing parameters such as activity units/mg protein, purity by SDS-PAGE, and absence of contaminating enzymatic activities. Production can adhere to ISO 9001 standards, with options for bulk manufacturing under GMP guidelines for diagnostic or pharmaceutical applications.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store frozen at -20°C or below in aliquots to avoid repeated freeze-thaw cycles. Under these conditions, the product remains stable for at least 12 months. The enzyme solution should be handled on ice during use.

Specification

Item	Specification
Appearance	Clear to slightly opalescent solution or lyophilized powder
Identification (Activity)	Catalyzes the conversion of Glucose-1-Phosphate to Glucose-6-Phosphate
Specific Activity	≥ 100 units/mg protein
Purity (SDS-PAGE)	≥ 90%
Protein Content (Biuret)	Contact for details
pH (in solution)	7.0 - 7.5
Stabilizers/Buffer	Phosphate or Tris buffer, may contain glycerol
Microbial Limits	< 100 CFU/g (for lyophilized grade)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.