Description

6-Phosphofructokinase Type Vii is a highly purified enzyme critical for catalyzing a key regulatory step in the glycolytic pathway. Its consistent activity and purity are essential for reliable results in biochemical research, diagnostic assay development, and metabolic studies. This product is primarily utilized by biotechnology firms, pharmaceutical R&D departments, and clinical diagnostic laboratories requiring precise enzymatic tools.

Application

• Biochemical Research & Assay Development: Used as a key reagent in enzymatic studies of glycolysis, metabolic flux analysis, and energy metabolism pathways.
• Diagnostic Kit Manufacturing: Serves as a critical component in the production of clinical diagnostic kits for measuring metabolites like fructose-6-phosphate or ATP.
• Pharmaceutical Screening: Employed in high-throughput screening (HTS) assays to identify potential modulators or inhibitors of glycolytic activity for drug discovery.
• Enzyme Kinetics Studies: Provides a standardized enzyme source for detailed kinetic analysis, determination of Michaelis constants (Km), and inhibitor studies.
• Educational & Reference Standards: Used in academic and industrial training laboratories as a reference standard for teaching enzymology principles.
• Metabolic Engineering: Applied in vitro to study and optimize synthetic metabolic pathways in engineered microorganisms.

Basic Information

Product Name	6-Phosphofructokinase Type Vii
CAS No.	9001-80-3
Molecular Formula	N/A (Enzyme)
Molecular Weight	~340 kDa (Tetramer, varies by source)
Synonyms	Phosphofructokinase 1; PFK-1; PFK; ATP-D-fructose-6-phosphate 1-phosphotransferase; Phosphohexokinase; 6-Phosphofructo-1-kinase; Type VII Phosphofructokinase; PFK Type VII from rabbit muscle; Glycolytic enzyme PFK
EINECS	232-625-8

Quality Control

Our 6-Phosphofructokinase Type Vii is produced and tested under stringent quality management systems. Each lot is characterized for specific activity, purity, and stability to ensure performance consistency for critical applications. A comprehensive Certificate of Analysis (COA) detailing assay results, protein content, and specific activity (U/mg protein) is provided with every shipment to guarantee traceability and compliance with your research or manufacturing protocols.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below. This material is hygroscopic (moisture-sensitive); allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake. Aliquot into working volumes upon receipt to avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	Lyophilized powder or suspension
Specific Activity	≥ 100 units/mg protein
Protein Content (Biuret)	≥ 90% of total
Purity (SDS-PAGE)	Single major band
Stabilizers	Potassium phosphate, EDTA, DTT (as applicable)
Solubility	Soluble in buffer or water
pH (in solution)	7.0 - 8.0

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.