Description

Diaphorase is a crucial oxidoreductase enzyme that catalyzes the transfer of electrons from NADH or NADPH to various electron acceptors, serving as a vital cofactor-regenerating agent. This makes it an indispensable component in diagnostic reagent formulations and biochemical research, ensuring accurate and efficient enzymatic cycling reactions. It is primarily utilized by manufacturers in the in-vitro diagnostics (IVD), pharmaceutical research, and life science research sectors.

Application

• Clinical Diagnostics: A key component in enzymatic assay kits for measuring substrates like glucose, cholesterol, triglycerides, and liver enzymes (ALT, AST) in blood serum.
• Biochemical Research: Used as an essential coupling enzyme in dehydrogenase-based assays to regenerate NAD+ from NADH, enabling continuous reaction monitoring.
• Pharmaceutical Development: Employed in high-throughput screening (HTS) platforms and enzyme activity studies for drug discovery and metabolic pathway analysis.
• Food Safety Testing: Integrated into biosensor and enzymatic methods for the detection of specific analytes or contaminants.
• Enzyme Engineering: Serves as a model enzyme in studies focusing on electron transfer mechanisms and oxidoreductase stability.

Basic Information

Product Name	Diaphorase
CAS No.	9001-68-7
Molecular Formula	Protein (Enzyme)
Molecular Weight	Approx. 50,000 - 60,000 Da (Contact for details)
Synonyms	Diaphorase (NADH); NADH Dehydrogenase; NADH-Diaphorase; Lipoamide Dehydrogenase (NAD+); Dihydrolipoamide Dehydrogenase; EC 1.8.1.4; Heart Diaphorase
EINECS	Contact for details

Quality Control

Our Diaphorase is produced and tested under stringent quality management systems to ensure batch-to-batch consistency and performance reliability for critical diagnostic and research applications. Each lot is characterized for specific activity, purity, and stability. A comprehensive Certificate of Analysis (COA) detailing assay results, including activity (U/mg protein), protein content, and purity profile, is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature before opening to minimize moisture absorption. Aliquot reconstituted solutions to avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	Lyophilized powder or solution
Identification (IR)	Conforms
Specific Activity	≥ 500 U/mg protein (Contact for specific grade)
Protein Content (Biuret)	≥ 90%
Purity (SDS-PAGE)	Single major band
Solubility	Clear, colorless solution in buffer
pH (1% solution)	6.0 - 8.0
Loss on Drying	≤ 5.0 %

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.