Description

Leucine Aminopeptidase, Cytosol From Hog Kidney, ~100 U/Mg is a highly purified enzyme preparation derived from porcine kidney cytosol, characterized by its specific activity of approximately 100 units per milligram of protein. This enzyme is critical for the selective cleavage of leucine and other hydrophobic amino acids from the N-terminus of peptides and proteins, serving as an essential tool in biochemical research and diagnostic applications. It is primarily utilized by research institutions, diagnostic kit manufacturers, and biotechnology companies engaged in proteomics, peptide sequencing, and enzyme kinetics studies to ensure precise and reliable analytical results.

Application

• Biochemical Research & Proteomics: Used for the specific hydrolysis of N-terminal leucine residues in peptide mapping and protein sequencing studies.
• Diagnostic Assay Development: Serves as a key component in the formulation of clinical diagnostic kits for measuring aminopeptidase activity in biological samples.
• Enzyme Kinetics & Inhibition Studies: Employed as a standard enzyme to study catalytic mechanisms, substrate specificity, and to screen for potential enzyme inhibitors.
• Food Science & Quality Control: Applied in research to analyze protein hydrolysis and assess flavor development in food products.
• Pharmaceutical Development: Utilized in the characterization of biopharmaceuticals, including peptide drugs and antibodies, to assess stability and degradation profiles.

Basic Information

Product Name	Leucine Aminopeptidase, Cytosol From Hog Kidney, ~100 U/Mg
CAS No.	9001-61-0
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	LAP; Leucine Aminopeptidase (Cytosol); L-Leucine Aminopeptidase; Aminopeptidase L; Cytosol Aminopeptidase; EC 3.4.11.1; Leucinaminopeptidase; Leucyl Aminopeptidase
EINECS	Contact for details

Quality Control

Our Leucine Aminopeptidase is produced and tested under stringent quality management protocols to ensure batch-to-batch consistency and high enzymatic activity. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing key parameters such as specific activity, protein content, and purity. We adhere to relevant quality guidelines suitable for research and diagnostic use, ensuring the material meets the exacting standards required for sensitive applications.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C. This product is hygroscopic (moisture-sensitive). For long-term stability, avoid repeated freeze-thaw cycles and store desiccated.

Specification

Item	Specification
Appearance	Lyophilized powder or solution
Specific Activity	~100 U/mg protein
Protein Content	Determined by Biuret or Bradford assay
Identification (IR/Activity)	Conforms
Purity (SDS-PAGE)	Major band at ~54 kDa
pH (in solution)	7.0 - 8.0
Solubility	Soluble in water or buffer

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.